An analysis of the role of protein kinase PKN3 in the progress of cancer and a development of new anticancer agent

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We prepared protein kinase PKN3 knockout mice to analyze the function of PKN3 in vivo. PKN3 knockout mice did not show any significant macroscopic morphological abnormalities and problems in reproductive potential and the feeding behavior. We isolated embryonic fibroblasts from PKN3 knockout mice and examined the wound healing activity and RasV12 induced focus forming activity, comparing with the wild type mice. And we crossed PKN3 knockout mice with pten conditional knockout mice which are susceptible to malignant neoplasms, and examined the effect of PKN3 on the tumorigenesis and metastasis. In order to prepare a specific inhibitor for PKN3, we prepared various deletion mutants and point mutants of PKN3 and analyzed the activation mechanism of PKN3 by checking the protein phosphorylation activity of the enzyme. We also checked the effect of PDK1 on the stability and the activation mechanism of PKN3.