Analysis of Physical Functions of the Novel Onoogene h WAPL, and its Applications in Diagnosis and Therapy

1.定量检测hWAPL基因表达水平建立宫颈癌诊断新体系目前，我们正在努力建立检测hWAPL基因表达水平的最佳定量方法。另一方面，由于HPVE6/E7癌蛋白诱导hWAPL的表达，我们开发了一种新的方法来确定宫颈标本中感染的HPV亚型(Oikawa，et.2.宫颈癌分子靶向治疗的新进展以前，我们发现hWAPL是宫颈癌基因治疗的有希望的靶点之一。目前，活性siRNA分配方法的最佳条件正在研究中。3.hWAPLOur研究的物理功能分析表明，hWAPL的非程序性过表达干扰了有丝分裂和胞质分裂，并通过诱导染色体不稳定(CIN)而促进肿瘤进展(Ohbayashi，Oikawa等)。Al.生物化学。生物群落。研究报告，2007,356：699-704)。此外，我们还发现hWAPL基因编码大量的选择性剪接变异体(Oikawa，et.Al.已提交)。尽管尚需进一步研究，但hWAPL变异体的表达模式可能是宫颈病变的一些临床标志物。

1.Development of a novel diagnostic system for uterine cervical carcinoma by quantification of the hWAPL mRNA expression levelUp to now, we are trying to establish the optimum quantification method for the hWAPL mRNA expression level. On the other hand, because HPV E6/E7 oncoproteins induce hWAPL expression, we have developed a novel protocol to determine the subtypes of HPVs infected in cervical samples (Oikawa, et. Al., submitted).2.Development of a novel molecular targeted therapy of uterine cervical carcinomaPreviously, we have found that hWAPL is one of the promising targets for a gene therapy of uterine cervical cancer. Now, the optimum conditions for the active siRNA dispencing method are under examination.3.Analysis of physical functions of hWAPLOur study suggested that unscheduled overexpression of hWAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability (CIN) (Ohbayashi, Oikawa, et. al., Biochem. Biophys. Res. Commun., 2007, 356 : 699-704). In addition, we found that the hWAPL gene encodes a large number of alternative spliced variants (Oikawa, et. Al., submitted). Although further studies are required, expression pattern of hWAPL variants may be some clinical markers for cervical lesions.