Regulation of T cell development mediated by BAF complex and Runx3

BAF 复合物和 Runx3 介导的 T 细胞发育调节

• 批准号: 18590477
• 负责人: SATO Takehito
• 金额: $ 2.57万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590477/
• 关键词: epigenetics; thymus; Runx3; Tcell; chromatin; BAF complex; 細胞系列決定; DNA複製; BAF; Runx; 分化

Developing thymocytes provide a useful tool to investigate the changes of the chromatin structure in the differentiation process. Based on the previous result that Runx3 plays pivotal roles in the development of CD8 thymocytes (Sato T., et. Al. Immunity 2005), this study has been designed to clarify the molecular basis for the lineage choice to CD8 thymocytes through the function of Runx3.This study revealed that chromatin re-modeler BAF complex controls CD4 and 8 expression quite similarly to Runx3. This raised the possibility that Runx3 binds to BAF complex and coordinately regulate CD4 and 8 expressions. In the study, I found that the control of DNA replication play some important roles in the lineage determination process. Blockade of DNA replication inhibited CD8 thymocytes differentiation, whereas augmentation of DNA replication inhibited CD4 thymocytes differentiation. Since DNA replication is accompanied with dynamic change of chromatin structure, it is to be clarified how DNA replication control affect on the lineage determination process in near future.

发育中的胸腺细胞为研究分化过程中染色质结构的变化提供了有用的工具。基于Runx3在CD8胸腺细胞发育中起关键作用的先前结果（Sato T.，et. Al.Immunity 2005），本研究旨在阐明通过Runx3的功能对CD8胸腺细胞谱系选择的分子基础。本研究揭示染色质重构剂BAF复合物与Runx3非常相似地控制CD4和8表达。Runx3可能与BAF复合物结合，协同调节CD4和CD8的表达。在研究中，我发现DNA复制的控制在谱系决定过程中起着重要的作用。阻断DNA复制抑制CD8胸腺细胞分化，而增强DNA复制抑制CD4胸腺细胞分化。由于DNA复制伴随着染色质结构的动态变化，因此DNA复制调控对谱系决定过程的影响有待进一步研究。

项目成果

胸腺における増殖制御と細胞系列の決定

胸腺中的生长控制和细胞谱系测定

• 发表时间: 2007
• 作者: 佐藤 健人;他
• 通讯作者: 他

Runx proteins are involved in regulation of CD122,Ly49 family and IF N-g expression during NK cell differentiation

Runx 蛋白参与 NK 细胞分化过程中 CD122、Ly49 家族和 IF N-g 表达的调节

• 发表时间: 2008
• 期刊: Internationl Immunology 20
• 作者: Ohno S;Sato T;et. al.
• 通讯作者: et. al.

Runx3はDNA複製コントロールを介してCD4/8 T細胞への系列決定に関与する

Runx3 通过控制 DNA 复制参与 CD4/8 T 细胞的谱系定型

• 发表时间: 2007
• 作者: 佐藤 健人;他
• 通讯作者: 他

Pleiotropic roles of Runx transcription factors in the differentiation and function of T lymphocytes

• DOI: 10.2174/157339508784325073
• 发表时间: 2008-05-01
• 期刊: Current Immunology Reviews
• 作者: Kohu, Kazuyoshi;Kubo, Masato;Satake, Masanobu
• 通讯作者: Satake, Masanobu

Pleiotropic Roles of Runx Transcription Factors in the Differenhation and Function of T Lymphocytes

Runx转录因子在T淋巴细胞分化和功能中的多效性作用

• 发表时间: 2008
• 期刊: Current Immunology Reviews 4(In press)
• 作者: Kohu K;Sato T;et. al.
• 通讯作者: et. al.