Understanding the pathophysiology of taste disorders by analysis of taste receptor gene expression in the tongue of patients with taste disorders.

通过分析味觉障碍患者舌头中味觉受体基因的表达来了解味觉障碍的病理生理学。

• 批准号: 18590541
• 负责人: IKEDA Minoru
• 金额: $ 2.44万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590541/
• 关键词: taste-related gene; T2R Family; taste disorder; human; taste disorder due to zinc deficiency; spontaneous abnormal taste

In this study, we examined the expression of ten genes (TAS2R40, 42, 43, 48, T2R3, 8, 9, 10, 13, and 16) related to the bitter taste receptor in healthy control subjects and in patients with taste disorders. I suggest changing the underlined to" Taste receptor gene expression was examined using RT-PCR. Total RNA was isolated from a sample scraped from the foliate papillae using TRIzol (Invitrogen). The total RNA was reverse transcribed using Superscript III (Invitrogen). The cDNA was subjected to PCR using ExTaq (Takara), and the PCR products were analyzed using a Bioanalyzer 2100 (Agilent).Patients with taste disorders showed a lower taste receptor gene expression than that of healthy control subjects, with TAS2R40 expressed at significantly lower levels. When considering the causes of taste disorders, cases of taste disorder due to zinc deficiency in particular tended to show lower expression of taste receptor genes. In these cases, TAS2R40 and T2R8 and 10 were expressed at significantly lower levels. TAS2R40 was expressed at significantly lower levels in cases of idiopathic taste disorder, whereas T2R3 showed significantly lower expression in cases with drug-induced taste disorder. Cases with bitter taste disorder showed lower expression of these taste genes compared to that of healthy control subjects. For TAS2R40, 42, T2R3,8 and 9, the lower expression was statistically significant.These results suggest that that TAS2R40, 42, T2R3, 8, 9and 10 are likely to be related to taste in humans. In addition, these findings can be applied to the diagnosis of taste disorders and in the determination of a prognosis. Clinical application appears most appropriate in cases with taste disorder due to zinc deficiency.

在这项研究中，我们检查了与健康对照组和味觉障碍患者中苦味受体有关的十个基因（TAS2R40、42、43、48、8、8、9、9、10、13和16）的表达。我建议使用rt-pcr更改下划线到“味觉受体基因表达。 （Agilent）患者的味觉受体表达低于健康对照受试者，当考虑到味道障碍的原因时，TAS2R40的表达较低在特发性味道障碍的病例中，在药物诱发的味觉障碍的病例中，T2R3的表达明显较低。与人类的味道有关。

项目成果

「増加している味覚障害-亜鉛との関連は解明されたか」。日常臨床における亜鉛の役割

“越来越多的味觉障碍——与锌的关系已被阐明吗？”

• 发表时间: 2007
• 作者: Nishihara;E.;et. al.;池田 稔
• 通讯作者: 池田 稔

味覚障害診療の手引き

味觉障碍治疗指南

• 发表时间: 2006
• 作者: 岡田 賢;他;池田 稔;菱沼 昭;池田 稔
• 通讯作者: 池田 稔

Causative factors of taste disorders and therapeutic effects of zinc agents among aged people.

老年人味觉障碍的病因及锌剂的治疗效果。

• 发表时间: 2008
• 期刊: The Journal of Laryngology & Otology 122
• 作者: Minoru Ikeda;Akihiro Ikui;Ayako Komiyama;Daisuke Kobayashi;Makoto Tanaka.
• 通讯作者: Makoto Tanaka.

Taste clinic - A summary : Causative factors and treatment of taste disorder. "Symposium : An approach to clinical taste disorders".

味觉诊所 - 总结：味觉障碍的病因和治疗。

• 发表时间: 2008
• 作者: M. Ikeda;H. Tomita
• 通讯作者: H. Tomita

The Story of Zinc-日常臨床における亜鉛の役割- 指名講演「増加している味覚障害-亜鉛との関連は解明されたか-」

锌的故事-锌在日常临床实践中的作用-提名讲座“味觉障碍增加-与锌的关系已被阐明吗？”

• 发表时间: 2008
• 作者: Hishinuma;A.;et. al.;池田 稔;池田 稔
• 通讯作者: 池田 稔