Development of in vitro NASH model using hepatocyte spheroid.

使用肝细胞球体开发体外 NASH 模型。

• 批准号: 18590735
• 负责人: SHIRAHA Hidenori
• 金额: $ 2.27万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590735/
• 关键词: hepatocellular spheroids; NASH; adipogenesis

Mouse fibroblast 3T3-L1 was able to be adipogenetic by IBMX and dexamethason under DMEM+FBS condition. We have tried to form spheroid with 3T3-L1. 3T3-L1 did not form spheroid under Williams E medium with insulin and EGF. To solve this problem, we tried two alternative ways. 1. To enhance EGF signaling, we utilized NIH3T3 cells with EGFR. These cells did not form spheroid. 2. To control cell-cell contact, a tumor suppresser gene was introduced into cells. Introduction of RUNX3 cDNA enhanced cell-cell contact of 3T3 cells. E-cadherin expression was increased in RUNX3 transfected cells.increased in RUNX3 transfected cells. While N-cadherin expression was decreased in these cells. RUNX3 introduced 3T3 was able to form spheroid. These cells may be a useful in vitro model for NASH.

在DMEM+FBS培养条件下，IBMX和地塞米松可诱导小鼠成纤维细胞3 T3-L1成脂。我们尝试用3 T3-L1形成球体。3 T3-L1在含胰岛素和EGF的威廉姆斯E培养基中不能形成球状体。为了解决这个问题，我们尝试了两种方法。1.为了增强EGF信号传导，我们利用具有EGFR的NIH 3 T3细胞。这些细胞不形成球状体。2.为了控制细胞间的接触，将肿瘤抑制基因引入细胞中。RUNX 3 cDNA的引入增强了3 T3细胞的细胞-细胞接触。E-cadherin表达在RUNX 3转染的细胞中增加。而N-cadherin表达减少。RUNX 3引入3 T3能够形成球状体。这些细胞可能是一个有用的NASH体外模型。

项目成果

Panic value in laboratory data(in Japanese)

实验室数据中的恐慌值（日文）

• 发表时间: 2006
• 期刊: Chugai Igaku
• 作者: Shinji;T.;Koide;N
• 通讯作者: N

Oral administration of taurine improves experimental pancreatic fibrosis.

口服牛磺酸可改善实验性胰腺纤维化。

• 发表时间: 2007
• 期刊: Journal of Gastroenterology and Hepatology 23(2)
• 作者: Shirahige A;Koide N;et. al.
• 通讯作者: et. al.

RUNX3 inhibits cell migration in hepatocellular carcinoma cell line.

RUNX3 抑制肝细胞癌细胞系中的细胞迁移。

• 发表时间: 2007
• 作者: Shigetomi Tanaka;Hidenori Shiraha;et. al.
• 通讯作者: et. al.

Cimetidine inhibits epidermal growth factor-induced cell signaling

• DOI: 10.1111/j.1440-1746.2006.04541.x
• 发表时间: 2007-03-01
• 期刊: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
• 影响因子: 4.1
• 作者: Fujikawa, Tatsuya;Shiraha, Hidenori;Shiratori, Yasushi
• 通讯作者: Shiratori, Yasushi

Three type 6 hepatitis C virus subgroups among blood donors in the Yangon area of Myanmar are identified as subtypes 6m and 6n, and a novel subtype by sequence analysis of the core region.

• DOI: 10.18926/amo/30719
• 发表时间: 2006-12
• 期刊: Acta medica Okayama
• 影响因子: 0.5
• 作者: Toshiyuki Shinji;A. Lwin;Katsunori Gokan;Mikako Obika;Hiromasa Ryuko;M. Khin;S. Okada;N. Koide