Prevention of spiral wave formation and breakup bycontmlof intracellular calcium dynamics

通过控制细胞内钙动态防止螺旋波的形成和破裂

• 批准号: 18590767
• 负责人: HONJO Haruo
• 金额: $ 2.49万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590767/
• 关键词: Arrhythmia; Action potential; Calcium dynamics; Optical mapping; Reentry; Elecronhvsioloey; 致死性不整脈; 蛍光プローブ; カルシウム; 蛍光色素

Recent theoretical studies using computer simulation has suggested that intercellular Can dynamics plays an important role in the initiation and maintenance of spiral wave reentry, which is a principal mechanism underlying ventricular tachyarrhythmias such as ventricular fibrillation/tachycardia. In this study, we have developed 2 types of dual optical mapping systems to record action potential/intracellular Ca^2+ fluorescence images simultaneously from isolated heart preparations of experimental animals: the first system has a dicrotic mirror to separate fluorescence signals and 2 high-speed CMOS video cameras, and the second system includes a high-speed switching apparatus of excitation LEDs combined with rotating optical filters fir fluorescence. A series of fluorescence images for action potentials and intracellular Ca^2+ were recorded simultaneously from the left ventricular epicardial myocardium Langendorff-perfused rabbit hearts with reasonable spatiotemporal resolution. In the firmer system, however, an additional development of image processing software to adjust the dual fluorescence images will be required, and in the latter system, an improvement of time resolution (20 frames/s) will be needed for precise analysis of relationship between action potentials and intracellular Ca^2+ dynamics to elucidate the roles of intracellular Ca^2+ dynamics on spiral wave reentry.

最近的计算机模拟理论研究表明，细胞间CaN动力学在螺旋波折返的启动和维持中起着重要作用，螺旋波折返是室性快速性心律失常(如室颤/心动过速)的主要机制。在这项研究中，我们开发了两种类型的双光学标测系统来同时记录实验动物心脏标本的动作电位/细胞内钙荧光图像：第一种系统具有分离荧光信号的分光镜和两个高速CMOS摄像机；第二种系统包括高速激发LED开关和旋转滤光片用于红外荧光。以合理的时空分辨率同时记录了Langendorff灌流的兔左室心外膜心肌的动作电位和细胞内钙离子的荧光图像。然而，在更稳定的系统中，需要另外开发图像处理软件来调整双荧光图像，而在后一种系统中，将需要提高时间分辨率(20帧/S)来精确分析动作电位和细胞内钙动力学之间的关系，以阐明细胞内钙动力学在螺旋波再入中的作用。

项目成果

Mechanisms of destabilization and early termination of Spiral wave reentry in the ventricle by a class III antiarrhyhtmic agent

III类抗心律失常药导致心室螺旋波折返不稳定和提前终止的机制

• 发表时间: 2007
• 期刊: Am J Physiol Heart Cite Physiol 292
• 作者: Yamazaki;M;et. al.
• 通讯作者: et. al.

Pharmacological control of spiral wave reentry in the ventricle : high-resolution optical mapping analysis (in Japanese)

心室螺旋波折返的药理学控制：高分辨率光学映射分析（日语）

• 发表时间: 2006
• 期刊: Igakunoayumi 217
• 作者: Kadama;I;et. al.
• 通讯作者: et. al.

膜電位及びカルシウムイオン動態同時マッピングシステムの開発に関する基礎的研究

膜电位和钙离子动力学同步作图系统开发的基础研究

• 发表时间: 2007
• 作者: 櫻井輝子;他
• 通讯作者: 他

Mechanisms of destabilization and early termination of spiral wave reentry in the ventricle by a class III antiarrhythmic agent, nifekalant

• DOI: 10.1152/ajpheart.00640.2006
• 发表时间: 2007-01-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
• 影响因子: 4.8
• 作者: Yamazaki, Masatoshi;Honjo, Haruo;Kodama, Itsuo
• 通讯作者: Kodama, Itsuo

Pharmacological control of spiral wave reentry in the ventricle : usefulness and limitation of K channel blockers (in Japanese)

心室螺旋波折返的药理学控制：K 通道阻滞剂的有用性和局限性（日语）

• 发表时间: 2007
• 期刊: Japanese Journal of Electrocardiology 27
• 作者: Kodama;I;et. al.
• 通讯作者: et. al.