Pathogenesis of Saunpus and roylmus assoaated bronchioitis obliterancee (SABO)

Saunpus 和 Roylmus 相关闭塞性细支气管炎 (SABO) 的发病机制

• 批准号: 18590845
• 负责人: IMAIZUMI Kazuyoshi
• 金额: $ 2.64万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590845/
• 关键词: Bronchiolitis obliterence; Sauropus Androgynus; TNF-a; monocytic lineage cell; endothelial cells; tracheal allograft; aqueous fraction; chromatography; 閉塞性細気管支炎; TNF-a; 薄層クロマトグラフィー

Recently, BO has been reported to develop following ingestion of Sauropus androgynus(SA), a leafy shrub distributed in Southeast Asia. However, little is known about direct effects of SA on airway resident cells or hematopoietic cells in vitro. In this study, we investigated the cytokines/chemokines production from monocytic lineage cells, epithelial cells and endothelial cells incubated with SA or fractions extracted from SA. Dry powder of SA was extracted with four solvents(hexan, acetone, methanol and water) and partitioned into fractions. Monocytic tumor cell lines(THP-1, RAW), human monocytes, alveolar macrophages, alveolar epithelial cell line(A549) and endothelial cell line(MSI)were incubated with SA solution or fractions eluted from SA. Among fractions from SA, only aqueous fraction induced significant increase of TNF- a, IP-10, MIG, MDC, IL-8 and VEGF production from monocytic lineage cells. The aqueous fraction of SA also suppressed the proliferation and induced apoptosis of endothelial cells but not of alveolar epithelial cells. In addition, the aqueous fraction enhanced luminal obliteration of mouse tracheal allograft model(in vivo model). For further partition of the aqueous fraction, high performance liquid chromatography was performed. A fraction with moderate polarity could induce significant TNF- a production from monocytic lineage cells. These results demonstrate that the aqueous fraction of SA contains substances that may play an important role for the pathogenesis of BO

最近，据报道，BO在摄入在东南亚分布的多叶灌木后摄入后发展。但是，对于SA对气道驻留细胞或造血细胞的直接影响知之甚少。在这项研究中，我们研究了单核细胞细胞，上皮细胞和与SA或从SA提取的馏分一起孵育的细胞因子/趋化因子产生。用四个溶剂（己糖，丙酮，甲醇和水）提取干粉末，并分成馏分。单核细胞肿瘤细胞系（THP-1，RAW），人单核细胞，肺泡巨噬细胞，肺泡上皮细胞系（A549）和内皮细胞系（MSI）与SA溶液或从SA洗脱的馏分一起孵育。在SA的馏分中，只有水分会引起TNF-A，IP-10，MIG，MDC，IL-8和VEGF产生的单核细胞谱系细胞的显着增加。 SA的水分也抑制了内皮细胞的增殖和诱导的凋亡，但不能抑制肺泡上皮细胞的凋亡。另外，水分分数增强了小鼠气管同种异体移植模型的腔内闭塞（体内模型）。为了进一步分割水分，进行了高性能液相色谱。具有中等极性的部分可能会引起单核细胞细胞产生的显着TNF-a。这些结果表明，SA的水分部分包含可能对BO发病机理起重要作用的物质

项目成果

Individual venation of effectiveness of midazolam as a sedative agent during bronchoscopy

支气管镜检查期间咪达唑仑作为镇静剂有效性的个体静脉分析

• 发表时间: 2007
• 作者: Hashimoto;I;et. al.
• 通讯作者: et. al.

Characterization of purified lung metastatic clone from heterogeneous cancer cluster

来自异质性癌症簇的纯化肺转移克隆的表征

• 发表时间: 2007
• 作者: Takagi;Y;et. al.
• 通讯作者: et. al.

進行性の呼吸不全と多臓器転移を来した胸膜原発epithelioid hemangioendo the lioma(EHE)の1例

原发于胸膜的上皮样血管瘤（EHE）伴进行性呼吸衰竭并转移至多个器官一例

• 发表时间: 2006
• 作者: Hashimoto;N;et. al.;Hashimoto I.;Takagi Y.;Matsuo M.;Hashimoto N.;今泉和良
• 通讯作者: 今泉和良

非小細胞肺癌におけるHDM2遺伝子の発現および遺伝子増幅とそのプロモーター領域の遺伝子多型に関する検討

非小细胞肺癌中HDM2基因表达、基因扩增及其启动子区遗传多态性研究

• 发表时间: 2006
• 作者: Takahashi;K;et. al.;小熊哲也;指尾豊和;伊藤理;武田直也;伊藤康;橋本泉;近藤征史
• 通讯作者: 近藤征史

Expression of macrophage-derived chemokine(MDC)/CCL22 in human lung cancer. Cancer Immunol Immunother.

巨噬细胞源性趋化因子（MDC）/CCL22在人肺癌中的表达。

• 发表时间: 2006
• 期刊: Cancer Immunol Immunother. 55
• 作者: Awaya;T.;et. al.;Nakanishi T
• 通讯作者: Nakanishi T