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Pathogenesis of Saunpus and roylmus assoaated bronchioitis obliterancee (SABO)

Saunpus 和 Roylmus 相关闭塞性细支气管炎 (SABO) 的发病机制

基本信息

批准号：
18590845
负责人：
IMAIZUMI Kazuyoshi
金额：
$ 2.64万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590845/
关键词：
Bronchiolitis obliterence Sauropus Androgynus TNF-a monocytic lineage cell endothelial cells tracheal allograft aqueous fraction chromatography 閉塞性細気管支炎 TNF-a 薄層クロマトグラフィー

项目摘要

Recently, BO has been reported to develop following ingestion of Sauropus androgynus(SA), a leafy shrub distributed in Southeast Asia. However, little is known about direct effects of SA on airway resident cells or hematopoietic cells in vitro. In this study, we investigated the cytokines/chemokines production from monocytic lineage cells, epithelial cells and endothelial cells incubated with SA or fractions extracted from SA. Dry powder of SA was extracted with four solvents(hexan, acetone, methanol and water) and partitioned into fractions. Monocytic tumor cell lines(THP-1, RAW), human monocytes, alveolar macrophages, alveolar epithelial cell line(A549) and endothelial cell line(MSI)were incubated with SA solution or fractions eluted from SA. Among fractions from SA, only aqueous fraction induced significant increase of TNF- a, IP-10, MIG, MDC, IL-8 and VEGF production from monocytic lineage cells. The aqueous fraction of SA also suppressed the proliferation and induced apoptosis of endothelial cells but not of alveolar epithelial cells. In addition, the aqueous fraction enhanced luminal obliteration of mouse tracheal allograft model(in vivo model). For further partition of the aqueous fraction, high performance liquid chromatography was performed. A fraction with moderate polarity could induce significant TNF- a production from monocytic lineage cells. These results demonstrate that the aqueous fraction of SA contains substances that may play an important role for the pathogenesis of BO

最近，据报道，BO是在摄入分布于东南亚的多叶灌木守宫木（SA）后发生的。然而，很少有人知道SA对气道驻留细胞或造血细胞在体外的直接影响。在这项研究中，我们研究了单核细胞谱系细胞，上皮细胞和内皮细胞与SA或从SA提取的馏分孵育的细胞因子/趋化因子的生产。SA干粉用四种溶剂（正己烷、丙酮、甲醇和水）提取，并分配成级分。单核细胞肿瘤细胞系（THP-1，RAW）、人单核细胞、肺泡巨噬细胞、肺泡上皮细胞系（A549）和内皮细胞系（MSI）与SA溶液或从SA洗脱的级分一起孵育。在来自SA的组分中，只有水组分诱导单核细胞谱系细胞的TNF-α、IP-10、TNF α、MDC、IL-8和VEGF产生显著增加。SA的水溶性组分也抑制了内皮细胞的增殖和诱导凋亡，但不诱导肺泡上皮细胞的凋亡。此外，水性部分增强小鼠气管同种异体移植物模型（体内模型）的管腔闭塞。为了进一步分配水性级分，进行高效液相色谱。具有中等极性的组分可诱导单核细胞系细胞产生显著的TNF-α。这些结果表明，SA的水部分含有的物质，可能发挥重要作用的发病机制BO