Pathogenesis of Saunpus and roylmus assoaated bronchioitis obliterancee (SABO)

最近，有报道称，在食用一种分布在东南亚的多叶灌木——雌雄同体索罗普斯（saropus androgynus）后发生BO。然而，SA对体外气道驻留细胞或造血细胞的直接影响尚不清楚。在这项研究中，我们研究了单核细胞系细胞、上皮细胞和内皮细胞与SA或SA提取物孵育后细胞因子/趋化因子的产生。用己烷、丙酮、甲醇和水四种溶剂提取白藜芦醇干粉，并对其进行分馏。单核细胞肿瘤细胞系（THP-1， RAW）、人单核细胞、肺泡巨噬细胞、肺泡上皮细胞系（A549）和内皮细胞系（MSI）用SA溶液或从SA中洗脱的组分孵育。在SA各组分中，只有水组分诱导单核细胞系细胞产生TNF- a、IP-10、MIG、MDC、IL-8和VEGF显著增加。SA水溶液组分还能抑制内皮细胞的增殖和诱导凋亡，但对肺泡上皮细胞无抑制作用。此外，水相组分增强了小鼠气管移植模型（体内模型）的腔隙闭塞性。为了进一步划分水馏分，采用高效液相色谱法。中等极性的部分可以诱导单核细胞产生显著的TNF- A。这些结果表明，SA的水相部分含有可能在BO发病机制中起重要作用的物质

Recently, BO has been reported to develop following ingestion of Sauropus androgynus(SA), a leafy shrub distributed in Southeast Asia. However, little is known about direct effects of SA on airway resident cells or hematopoietic cells in vitro. In this study, we investigated the cytokines/chemokines production from monocytic lineage cells, epithelial cells and endothelial cells incubated with SA or fractions extracted from SA. Dry powder of SA was extracted with four solvents(hexan, acetone, methanol and water) and partitioned into fractions. Monocytic tumor cell lines(THP-1, RAW), human monocytes, alveolar macrophages, alveolar epithelial cell line(A549) and endothelial cell line(MSI)were incubated with SA solution or fractions eluted from SA. Among fractions from SA, only aqueous fraction induced significant increase of TNF- a, IP-10, MIG, MDC, IL-8 and VEGF production from monocytic lineage cells. The aqueous fraction of SA also suppressed the proliferation and induced apoptosis of endothelial cells but not of alveolar epithelial cells. In addition, the aqueous fraction enhanced luminal obliteration of mouse tracheal allograft model(in vivo model). For further partition of the aqueous fraction, high performance liquid chromatography was performed. A fraction with moderate polarity could induce significant TNF- a production from monocytic lineage cells. These results demonstrate that the aqueous fraction of SA contains substances that may play an important role for the pathogenesis of BO