Exploration of new therapy and diagnosis in lymph metastasis of lung cancer based on theprofile of lymphangiogenic-related genes expression

基于淋巴管生成相关基因表达谱探索肺癌淋巴转移的新疗法和诊断

• 批准号: 22590857
• 负责人: IMAIZUMI Kazuyoshi
• 金额: $ 2.83万
• 依托单位: Fujita Health University (2011-2012)Nagoya University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590857/
• 关键词: 呼吸器病学; 肺がん; リンパ節; EMT; HIF1α; VEGF; 内科; 呼吸器内科; 肺癌; リンパ節転移

Based on our recognition for the importance of tumor microenvironment(TM), TM induces acquisition of malignant phenotype such as epithelial-mesenchymal transition (EMT), resulting in metastasis into lymph nodes. In this study, persistent tissue hypoxia might be involved in tumor tissue in an in vivo orthotopic intrapulmonary implantation model. Persistent hypoxia also induced the stabilized HIF1α expression in lung cancer cells. Transduction of HIF1α into lung cancers with the Doxycycline inducible gene expression system caused de novo VEGF-A expression. Taken together with the evidence of increasing VEGF receptor expression lymphatic endothelial cells in sentinel lymph nodes, hypoxia as TM in lung cancers might be a therapeutic target for the metastasis into lymph nodes and lymphangiogenesis.

基于我们对肿瘤微环境（TM）重要性的认识，TM诱导获得恶性表型，如上皮-间质转化（EMT），导致淋巴结转移。在这项研究中，持续的组织缺氧可能涉及在体内原位肺内植入模型中的肿瘤组织。持续低氧也可诱导肺癌细胞稳定表达HIF 1 α。用强力霉素诱导的基因表达系统将HIF 1 α导入肺癌引起VEGF-A的重新表达。结合前哨淋巴结中VEGF受体表达增加的淋巴管内皮细胞的证据，低氧作为TM在肺癌中可能是淋巴结转移和淋巴管生成的治疗靶点。

项目成果

Modulated expression of surfactant protein D in acute lung injury might be associated with epitherial-mesenchymal transition in epithelial cells

急性肺损伤中表面活性蛋白 D 的表达调节可能与上皮细胞的上皮间质转化有关

• 发表时间: 2011
• 作者: Takahashi Y;Tamakoshi K;Matsushima M;Kawabe T.;D Aoyama
• 通讯作者: D Aoyama

Endobronchial ultrasound transbronchial needle aspiration in older people

• DOI: 10.1111/ggi.12043
• 发表时间: 2013-10-01
• 期刊: GERIATRICS & GERONTOLOGY INTERNATIONAL
• 影响因子: 3.3
• 作者: Okachi, Shotaro;Imai, Naoyuki;Hasegawa, Yoshinori
• 通讯作者: Hasegawa, Yoshinori

Attenuation of TGF-ss-Stimulated Collagen Production in Fibroblasts by Quercetin-Induced HO-1.

槲皮素诱导的 HO-1 减弱成纤维细胞中 TGF-β 刺激的胶原蛋白产生。

• 发表时间: 2011
• 期刊: Am J Respir Cell Mol Biol
• 作者: Nakamura T;Matsushima M;Hayashi Y,Shibasaki M;Imaizumi K;Hashimoto N;Shimokata K;Hasegawa Y;Kawabe T
• 通讯作者: Kawabe T

Detection of loci for allergic asthma using SMXA recombinant inbred strains of mice

• DOI: 10.1007/s00251-012-0656-7
• 发表时间: 2012
• 期刊: Immunogenetics
• 影响因子: 3.2
• 作者: T. Ohno;M. Okamoto;T. Hara;N. Hashimoto;K. Imaizumi;M. Matsushima;M. Nishimura;K. Shimokata;Y. Hasegawa;T. Kawabe

EBUS-GS併用経気管支鏡下生検で肺クリプトコッカス症と診断された３症例の検討

经支气管镜联合EBUS-GS诊断肺隐球菌病3例研究

• 发表时间: 2012
• 作者: Ishida S;Nishizawa N;Kitatsuji M;Ohshima H;Hasegawa Y;Matsushima M;Kawabe T;伊藤亮太
• 通讯作者: 伊藤亮太