Carcinogenesis in chronic epithelial damage of the lung

肺慢性上皮损伤的致癌作用

• 批准号: 18590869
• 负责人: KUDOH Shoji
• 金额: $ 2.53万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590869/
• 关键词: Chronic epithelium damage; Lung cancer; IPF; TGF-β; EMT; 発癌分子機構; cDNA; 抗体アレイ; 2D-DIGE

Patients with chronic epithelium damage have an increased risk of developing lung cancer. To identify key molecules involved in malignant transformation in these patients, we analyzed the expression profiles of lung tumor and lung tissue from these patients using cDNA arrays and real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Altered expressions of the genes downstream of transforming growth factor-β (TGF-β) were identified in tumor samples from lung cancer patients with IPF, in which TGF-β is overexpressed, using real-time RT-PCR. Our findings indicate that the inactivation of TGF-β signaling pathway may play an important role in pulmonary carcinogensis or progression of lung cancer in the patients with chronic epithelium damage. In addition, we analyzed the change of the expression profiles after TGF-β exposure in TGF-β-sensitive epithelial cells using cDNA array, 2D-DIGE, and Ab-array. Many molecules were identified, expression levels of which were increased or decreased after TGF-β exposure. One of those is concerned with EMT. Further study of the relationship of this molecule and Smads is required. These findings could be used to develop preventive measures or treatment of lung cancer patients with chronic epithelium damage.

慢性上皮损伤患者患肺​​癌的风险增加。为了鉴定这些患者中涉及恶性转化的关键分子，我们使用cDNA阵列和实时定量逆转录酶 - 聚合酶链（RT-PCR）分析了这些患者的肺肿瘤和肺组织的表达谱。在IPF肺癌患者的肿瘤样本中鉴定了转化生长因子-β（TGF-β）下游基因的表达改变，其中使用实时RT-PCR过表达TGF-β。我们的发现表明，TGF-β信号通路的失活可能在慢性上皮损伤患者中在肺癌或肺癌的进展中起重要作用。此外，我们使用cDNA阵列，2D-DIGE和AB-ARRAY分析了TGF-β暴露后TGF-β暴露后表达谱的变化。鉴定出许多分子，TGF-β暴露后的表达水平升高或降低。其中之一与EMT有关。需要进一步研究该分子和SMAD的关系。这些发现可用于开发预防性测量或治疗患有慢性上皮损伤的肺癌患者。

项目成果

当科における肺癌臨床検体のEGFR遺伝子変異についての検討

我科临床肺癌标本EGFR基因突变情况调查

• 发表时间: 2006
• 作者: Rintaro;Noro;et. al.;野呂 林太郎;弦間 昭彦;須藤 淳子;小斉平 聖治
• 通讯作者: 小斉平 聖治

TS-1特定使用成績調査(間質性肺炎)の検討結果について

关于TS-1特定使用结果调查结果（间质性肺炎）

• 发表时间: 2006
• 作者: Rintaro;Noro;et. al.;野呂 林太郎;弦間 昭彦
• 通讯作者: 弦間 昭彦

間質性肺炎合併肺癌の臨床的検討

肺癌并发间质性肺炎的临床研究

• 发表时间: 2006
• 作者: Rintaro;Noro;et. al.;野呂 林太郎;弦間 昭彦;須藤 淳子
• 通讯作者: 須藤 淳子

Plasma proteomics of lung cancer by a linkage of multi-dimensional liquid chrom atography and two-dimensional difference gel electrophoresis(2D-DIGE)

多维液相色谱与二维差异凝胶电泳（2D-DIGE）联动的肺癌血浆蛋白质组学

• 发表时间: 2006
• 期刊: Proteomics 6
• 作者: Tetsuya Okano;et. al.
• 通讯作者: et. al.

総合臨床

综合临床

• 发表时间: 2007
• 作者: 清家 正博;弦間 昭彦;弦間 昭彦
• 通讯作者: 弦間 昭彦