Effects of novel brain prothctants on neuroregeneration falbwing brain ischemia

新型脑保护剂对弱翼脑缺血神经再生的影响

基本信息

批准号：
18590958
负责人：
KATAYAMA Yasuo
金额：
$ 2.34万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Objects and Methods : Neuroregeneration and releasing neurotraphic factors have been considered as a mechanism for functional improvement after transplantation of bone marrow cells in experimental brain ischemia model. However protective effects of transplantation of bone marrow cells immediately after reperfusion have been rarely investigated. The present study examined, using rat transient focal ischemia model (90 min), different protective effects between intra-arterial and intravenous administration routs after transplantation of bone marrow mononuclear cells (BMMCs), which need no incubation period. Then, the present study sought to investigate whether an intravenous transplantation of bone marrow stromal cells (BMSCs), which require incubation period, has also protective effects in the same rat model, and whether a combined therapy with BMSC transplantation and immunosuppressant FK506 enhance such neuroprotection. Furthermore, distribution and differentiation of the transplanted … More BMSCs was also investigated, and longitudinal cellular dynamics of the transplanted cells using MRI.Results : Intra-arterial BMMC transplantation ameliorated infarct volume and improved functional scores, while intravenous BMMC transplantation had no such protective effects. The transplanted BMMCs were observed more frequently within the ischemic hemisphere in the intra-arterial administration group than the intravenous administration group. A combined therapy with intravenous BMSC transplantation and FK506 showed greater neuroprotection. The transplanted BMSCs were differentiated into neurons and astrocytes by one month after transplantation. MRI study revealed that the transplanted cells labeled with superparamagnetic iron oxide were observed on T2 weighted images, and that such cells were gradually decreased in number by one month after transplantation.Conclusions: The present study clearly showed that intra-arterial administration immediately after reperfusion potentiated the effective delivery of BMMCs to the brain compared to intravenous administration, and that FK506 combined with BMSC transplantation enhanced such neuroprotection, leading to a decrease in ischemic damage and good functional recovery in rat transient ischemia model. Efficient BMMC delivery to the brain and modification of transplantation circumstance may be important in clinical application of BMMC transplantation for the treatment of acute ischemic stroke. Less

物体和方法：在实验性脑缺血模型中骨髓细胞移植后，神经变成和释放神经图因子被认为是功能改善的机制。然而，很少研究在再灌注后立即受到骨髓细胞的保护作用。本研究使用大鼠瞬态局灶性缺血模型（90分钟）进行了检查，在移植骨髓单核细胞（BMMC）后，动脉内和静脉内给药途径之间进行了不同的保护作用，这些途径无需孵育期。然后，本研究感觉到需要研究需要孵育期的骨髓间质细胞（BMSC）的静脉移植是否也保护了同一大鼠模型中的作用，以及与BMSC移植和免疫抑制剂FK506的联合治疗是否是否增强了这种神经保护性。此外，还研究了移植的移植物的分布和分化……还研究了更多的BMSC，并使用MRI进行移植细胞的纵向细胞动力学：消除性BMMC移植缓解基础架构的基础结构和改善的功能评分，而静脉内BMMC的静脉输液bmmc bmmc bmmc却没有产生这种致命的效应。在动脉内给药组的缺血性半球中，观察到移植的BMMC比静脉内给药组更频繁。静脉内BMSC移植和FK506的联合治疗显示出更大的神经保护作用。移植后一个月，将移植的BMSC分化为神经元和星形胶质细胞。 MRI study revealed that the transplanted cells labeled with superparamagnetic iron oxide were observed on T2 weighted images, and that such cells were gradually improved in number by one month after transplantation.Conclusions: The present study clearly shown that Intra-arterial administration immediately after reperfusion potentiated the effective delivery of BMMCs to the brain compared to intravenous administration, and that FK506 combined with BMSC transplantation增强了这种神经保护性，导致缺血性损伤减少和大鼠短暂性缺血模型的功能恢复。有效的BMMC向大脑传递，移植情况的修饰对于临床应用BMMC移植以治疗急性缺血性中风。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Treatment using bone marrow stromal cells in rat brain ischemia model : neuroregeneration and neuroprotective effect

使用骨髓基质细胞治疗大鼠脑缺血模型：神经再生和神经保护作用

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Suda;S.;Shimazaki;K.;Ueda;M.;Kato;K.;Inaba;T.;Kamiya;N.;Nishiyama;Y.;Okubo;S.;Yokota;H.;Oguro;K.;Watanabe;H.;Katayama;Y
通讯作者：
Y

ラット局所脳虚血モデルにおける急性期自己骨髄単核球細胞投与の脳保護効果の検討

急性自体骨髓单个核细胞注射对大鼠局灶性脑缺血模型脑保护作用的研究