Analysis of the Ro52's function and structure and biological significance of autoimmune disease

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An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjogren's syndrome and systemiclupus erythematosus,. However, the physiological function of the autoantigen SS-A/Ro52 has not yet been elucidated. To investigate this function, we have studied the role of Ro52 protein in T cell activation.Then, we found that overexpression of SS-A/Ro52 in Jurkat T cell resulted in enhanced IL-2 production following CD28 stimulation. Moreover to investigate the mechanism of Ro52 signaling pathway, we searched Ro52 associated molecules. And, we identified human decapping enzyme 2 (hDCP2) as a binding protein with Ro52. Ro52 colocalized with hDCP2 in processing bodies (p-bodies) in 293 FT cells. We also demonstrated that the N-terminus and C-terminus of Ro52 bound to hDCP2 in a mammalian GST pull down assay system. Moreover, in vitro decapping assay revealed that Ro52 enhanced decapping activity of hDCP2, as well as upregulating hDCP2 expression. Our present data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation.