权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Small diameter vascular prosthesis with anti-clotting and anti-inflammatory response

具有抗凝血和抗炎反应的小直径人工血管

基本信息

批准号：
18591417
负责人：
SHIOZAKI Takahiro
金额：
$ 1.92万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Preserving endothelial function of arterial conduits may affect early and long-term patency of small caliber grafts. Mechanism of angiotensin II-induced endothelial dysfunction is not well understood. So we evaluated the in vitro effects of angiotensin II and valsartan (angiotensin II type 1 receptor blocker, ARB) on the endothelium dependent relaxation of aortic ring from wister rats. The aortas which were excised in anesthetized rats cut into ring segments. Each ring was incubated for 24 h at 37℃ in Dulbecco's modified Eagle's medium containing 0.5% calf serum with or without angiotensin II and valsartan. Using an organ bath technique, contractile responses to 80mmol/L KCl and 300nmol/L phenylephrine were evaluated. And relaxation responses were examined using sodium nitroprusside (10^<-11> to 10^<-7>mol/L) or acetylcholine (10^<-9> to 10^<-5> mol/L) after contraction by 300nmol/L phenylephrine. Contractile response to phenylephrine was potentiated by angiotensin II (100nmol/L). Angiotensin II (100nmol/L) responded to sodium nitroprusside, but impaired relaxations to acetylcholine. However, adding valsartan responded to acetylcholine. The endothelium-dependent relaxation of the aorta by means of valsartan was improved. We conclude that Varsartan improved the endothelium-dependent relaxation of the aorta in rats that received angiotensin II.

保留动脉导管内皮功能可能影响小口径移植物的早期和长期通畅。血管紧张素ii诱导内皮功能障碍的机制尚不清楚。因此，我们在体外研究了血管紧张素II和缬沙坦（血管紧张素II 1型受体阻滞剂，ARB）对大鼠主动脉环内皮依赖性舒张的影响。麻醉后切除的大鼠主动脉切成环状段。每个环在含有0.5%小牛血清（含或不含血管紧张素II和缬沙坦）的Dulbecco's modified Eagle's培养基中37℃孵养24 h。采用器官浴技术，对80mmol/L KCl和300nmol/L苯肾上腺素的收缩反应进行了评价。用300nmol/L的苯肾上腺素收缩后，用硝普钠（10^<-11>至10^<-7>mol/L）或乙酰胆碱（10^<-9>至10^<-5> mol/L）检测松弛反应。血管紧张素II （100nmol/L）增强了对苯肾上腺素的收缩反应。血管紧张素II （100nmol/L）对硝普钠有反应，但对乙酰胆碱的松弛作用受损。然而，加入缬沙坦对乙酰胆碱有反应。缬沙坦可改善主动脉内皮依赖性舒张。我们得出结论，缬沙坦改善了血管紧张素II大鼠主动脉内皮依赖性松弛。