Diagnostic demonstration of telomere measurement anaplastic cancer arose from differentiated cancer in the thyroid by tissue quantitative fluorescence in situ hybridization

组织定量荧光原位杂交端粒测量对甲状腺分化癌引起的间变性癌的诊断示范

• 批准号: 18591432
• 负责人: KAMMORI Makoto
• 金额: $ 2.57万
• 依托单位: Nihon University (2007)The University of Tokyo (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591432/
• 关键词: tissue O-FISE; Thyroid; Telomere; テロメラーゼ; 甲状腺癌; 組織Q-FISH法; 未分化転化

We have developed a novel method for evaluating telomere length using tissue quantitative fluorescence in situ hybridization (Q-FISH). Our method can be used to distinguish between benign and malignant tissue in esophageal lesions (Oncology, 2006). We demonstrated to evaluate telomere length in papillary carcinoma and normal thyroid tissue by Q-FISH. The average TCR in the papillary carcinoma cell was significantly lower than that in the follicular epithelial cell and fibroblast cells in the thyroid (P<0.05). The advanced papillary carcinoma of more than T2 (more than 2 cm) and Nlb (lateral area of metastasis of lymph-node) was significantly lower telomere length than the early papillary carcinoma of less than T2 and Nlb. These results suggested our TCR method can be used to distinguish between advanced and early carcinoma of the thyroid. On the other hand, one of the most aggressive human malignancies, anaplastic thyroid carcinoma (ATC), has an extremely poor prognosis that may be exp … More lained by its genomic instability. We hypothesized that the very rapid cell turnover observed in ATC might accelerate telomere shortening and chromosomal instability associated with tumor cell malignancy. To compare and measure chromosomal aberrations and telomere shortening in an anaplastic thyroid cancer cell line, OCUT-1, we applied quantitative fluorescence in situ hybridization (Q-FISH) techniques. OCUT-1 cells display several chromosomal abnormalities, but have a near-normal chromosome complement of 46, XX, making it easy to analyze the karyotype. The karyotype showed 50, XX, +7, +11, der(11)t(3 ; 11)(q23 ; q23)x2, del(12)(p11.2p12), +20, +1mar. We analyzed carefully the abnormalities in karyotype of OCUT-1 associated with telomere shortening on each chromosomes and expression of subtelomeres. Subtelomeres on the abnormal chromosome der(11)t(3 ; 11)(q23 ; q23)x2 also showed loss of signals on 11p, but there was no loss of signals in the cytogenetically normal trisomies 7 and 20 or the abnormal chromosome del(12)(p11.2p12). Subtelomeres of 3q had eight signals, one pair remaining in place on 3q and another pair on the abnormal 11p. Our findings suggest that telomere shortening and subtelomere loss are correlated with genetic instability in this anaplastic thyroid carcinoma cell line. Less

我们已经开发了一种新的方法来评估端粒长度使用组织定量荧光原位杂交（Q-FISH）。我们的方法可用于区分食管病变中的良性和恶性组织（Oncology，2006）。我们证明，评估端粒长度在乳头状癌和正常甲状腺组织的Q-FISH。甲状腺乳头状癌细胞的平均TCR显著低于滤泡上皮细胞和成纤维细胞（P<0.05）。大于T2（大于2cm）和Nlb（淋巴结转移区外侧）的晚期乳头状癌端粒长度显著低于小于T2和Nlb的早期乳头状癌。结果表明，TCR法可用于鉴别晚期和早期甲状腺癌。另一方面，甲状腺间变性癌（ATC）是最具侵袭性的人类恶性肿瘤之一，其预后极差，可能与甲状腺癌的预后有关。 ...更多信息 基因组的不稳定性我们推测，在ATC中观察到的非常快速的细胞更新可能会加速端粒缩短和与肿瘤细胞恶性相关的染色体不稳定性。为了比较和测量甲状腺间变性癌细胞系OCUT-1的染色体畸变和端粒缩短，我们应用定量荧光原位杂交（Q-FISH）技术。OCUT-1细胞显示出几种染色体异常，但具有接近正常的46，XX染色体补体，使其易于分析核型。核型为50，XX，+7，+11，der（11）t（3 ; 11）（q23 ; q23）x2，del（12）（p11.2p12），+20，+1mar。我们仔细分析了OCUT-1的核型异常与各染色体上端粒缩短和亚端粒表达的关系。异常染色体der（11）t（3 ; 11）（q23 ; q23）x2上的亚端粒也显示11 p信号丢失，但在细胞遗传学正常的7号和20号三体或异常染色体del（12）（p11.2p12）中没有信号丢失。3q的亚端粒有8个信号，一对留在3q上，另一对在异常的11 p上。我们的研究结果表明，端粒缩短和亚端粒丢失与甲状腺未分化癌细胞系的遗传不稳定性相关。少

项目成果

Telomere metabolism and diagnostic demonstration of telomere measurement in human esophagus for distinguishing benign from malignancy by tissue quantitative fluorescence in situ hybridization.

端粒代谢和人食管端粒测量的诊断示范，通过组织定量荧光原位杂交区分良恶性。

• 期刊: Oncology (in press)
• 作者: Kammori M;et al.
• 通讯作者: et al.

Specific subtelomere loss on chromosome der (11) t(3 ; 11) (q23 ; q23) x2 in anaplastic thyroid cancer cell line OCUT-1.

间变性甲状腺癌细胞系 OCUT-1 中染色体 der (11) t(3 ; 11) (q23 ; q23) x2 上的特异性亚端粒丢失。

• 发表时间: 2006
• 期刊: Int J Mol Med Jul ; 18(1)
• 作者: 堀倫 子;大橋 健一;その他;Hoshino M;Ishii A.;Yoshimi F;Kammori M.
• 通讯作者: Kammori M.

Telomere Metabolism and Diagnostic Demonstration of Telomere Measurement in the Human Esophagus for Distinguishing Benign from Malignant issue by Tissue Quantitative Fluorescence in situ Hybridization

人食管端粒代谢和端粒测量诊断示范，通过组织定量荧光原位杂交区分良恶性

• 发表时间: 2006
• 期刊: Oncology 71
• 作者: Makoto Kammori;et. al.
• 通讯作者: et. al.

組織Q-FISH法を用いた甲状腺乳頭癌のテロメア長解析

应用组织Q-FISH法分析甲状腺乳头状癌的端粒长度

• 发表时间: 2007
• 作者: Joh K;Taguchi T;Shigematsu H;Kobayashi Y;Sato H;Nishi S;Katafuchi R;Nomura S;Fujigaki Y;Utsunomiya Y;Sugiyama H;Saito T;Makino H;仲村賢一;笠原一郎;仲村賢一;城 謙輔;柏尾光彦
• 通讯作者: 柏尾光彦

甲状腺癌を用いた組織Q-FISH(Quantitative fluorescence in hybridization)によるテロメア長測定

使用甲状腺癌组织 Q-FISH（定量荧光杂交）测量端粒长度

• 发表时间: 2006
• 作者: 神森 眞;他
• 通讯作者: 他