Proteomic profiling of autoantibodies in patients with esophageal squamous cell carcinoma

食管鳞状细胞癌患者自身抗体的蛋白质组学分析

• 批准号: 18591465
• 负责人: TAKEDA Shigeru
• 金额: $ 2.57万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591465/
• 关键词: proteomics; Esophageal cancer; LC-MS; MS; Tropomyosin

To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired non-cancerous tissues by two-dimensional electrophoresis (2-DE) and tandem mass spectrometry. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by peptide mass fingerprinting (PMF) with liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin Al, tropomyosin beta chain, 14-3-3 protein sigma and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.

为了鉴定与食管癌发生相关的蛋白质，我们采用双向电泳（2-DE）和串联质谱法对16例食管鳞状细胞癌（ESCC）和配对的非癌组织进行了蛋白质谱分析。在癌组织中，三个点显示蛋白质量显著上调，而八个点显著下调。通过液相色谱-质谱/质谱（LC-MS/MS）的肽质量指纹图谱（PMF）确定斑点的身份，并通过免疫印迹法进行确认。表达上调的蛋白质为原肌球蛋白α 4链、转凝胶蛋白和丙酮酸激酶。下调的蛋白质为血清白蛋白前体、膜联蛋白A1亚型、原肌球蛋白β链、14-3-3蛋白σ和血清转铁蛋白前体亚型。在所有16例病例中，通过免疫印迹证实了原肌球蛋白α 4链的上调。原肌球蛋白α 4链在食管鳞癌细胞和邻近的成纤维细胞中的定位通过免疫组织化学证实。

项目成果

Proteomic analysis of autoantibodies in patients with hepatocellular carcinoma

• DOI: 10.1002/pmic.200500346
• 发表时间: 2006-07-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Takashima, Motonari;Kuramitsu, Yasuhiro;Nakamura, Kazuyuki
• 通讯作者: Nakamura, Kazuyuki

Analysis for Auoantibodies in Sera from esophagus Squamous Cell Carcinoma-Bearing Patients

食管鳞状细胞癌患者血清中抗体的分析

• 发表时间: 2007
• 作者: Toshio;Harada
• 通讯作者: Harada

高齢者食道癌における食道切除術症例の検討

老年食管癌切除术病例研究

• 发表时间: 2007
• 作者: Shigeru;Takeda;原田 俊夫;武田 茂;高島元成;蔵満保宏;武田 茂;武田茂;武田 茂
• 通讯作者: 武田 茂

Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry

• DOI: 10.1002/prca.200600609
• 发表时间: 2007-02-01
• 期刊: PROTEOMICS CLINICAL APPLICATIONS
• 影响因子: 2
• 作者: Harada, Toshio;Kuramitsu, Yasuhiro;Nakamura, Kazuyuki
• 通讯作者: Nakamura, Kazuyuki

A clinical study of esophagectomy after chemo-radiation therapy for advanted esophageal carcinoma

晚期食管癌放化疗后食管切除术的临床研究

• 发表时间: 2007
• 期刊: Gan To Kagaku Ryoho 34(12)
• 作者: Shigeru;Takeda;原田 俊夫;武田 茂
• 通讯作者: 武田 茂