权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Research of immunotherapy and splenectomy for liver dysfunction.

肝功能障碍的免疫治疗和脾切除研究。

基本信息

批准号：
18591506
负责人：
TSUKADA Kazuhiro
金额：
$ 2.49万
依托单位：
University of Toyama
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591506/
关键词：
Portal hypertension Splenomegaly Activated T lymphocyte 活性化型リンパ球

项目摘要

1. Making the animal experiment model of portal hypertension and splenomegaly.We tried to make an animal experiment model that was going to reproduce a liver damage, portal hypertension and splenomegaly with a rat experimentally. We used a carbon tetrachloride treated group, a bacillus mort treated group, a bovine serum albumin (BSA) treated group, mitogen treated group, Portocaval shunt (PCS) group, a portal vein ligation county and tested it. It was confirmed that splenomegaly was developed in the group that were ligated the portal vein with two phases.2. Clinical examination in portal hypertension casesWe adjusted lymphocyte from blood and spleen of portal hypertension patients that were performed splenectomy operation. And we analyzed the lymphocyte about expression of CD25, CD26, CD69, CD70 which was an activation marker by using the flowcyte meter. As a result, there were more activated T lymphocytes existed in preoperative blood of portal hypertension case, in comparison with control. In addition, a ratio of an early phase activated T lymphocyte was high in the extracted spleen. After operation, the ratio of the activated T lymphocyte of blood fell in transientness, but it increased again from three postoperative day. As a result, it was suggested, an early phase activated T lymphocyte was accumulated to the spleen in the portal hypertension patient, and after maturation, it flowed out into the peripheral blood.

1.门静脉高压症脾肿大动物实验模型的建立本实验拟用大鼠复制肝损伤、门脉高压、脾肿大的动物模型。分别用四氯化碳处理组、枯草杆菌处理组、牛血清白蛋白(BSA)处理组、丝裂原处理组、门腔分流术(PCS)组、门静脉结扎县进行检测。结果：1.分两期结扎门静脉组大鼠脾肿大。门脉高压症的临床检查我们对行脾切除手术的门脉高压症患者的血液和脾淋巴细胞进行了调节。用流式细胞仪检测淋巴细胞活化标志物CD25、CD26、CD69、CD70的表达。结果表明，门静脉高压症患者术前血中存在较多的活化T淋巴细胞。此外，在提取的脾中，早期激活的T淋巴细胞的比例很高。术后血液中活化的T淋巴细胞比例呈一过性下降，术后3d开始回升。提示门脉高压症患者早期活化的T淋巴细胞聚集在脾内，成熟后流入外周血。