Development of Techniques for Improvement of Silk Structure, Establishment of Mass Production, and Application to New Silk-based Materials for Bone and Teeth Regeneration

蚕丝结构改良技术开发、规模化生产及新型蚕丝骨牙齿再生材料的应用

• 批准号: 18105007
• 负责人: ASAKURA Tetsuo
• 金额: $ 71.8万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18105007/
• 关键词: 天然・生体高分子材料; トランスジェニック絹; 歯・骨再生; 高分子構造・物性; 生体材料; 移植・再生医療; 蛋白質; 絹

15 silk-like materials for tooth and bone regeneration were designed and produced by transgenic silkworm. Then the porous silk samples with different biodegradable characters were developed. Through in vitro evaluation by the cell cultivation and in vivo evaluation with animal implantation, the excellent materials for tooth and bone regeneration were developed on the basis of silk.

通过转基因蚕设计和生产15种用于牙齿和骨骼再生的丝绸样材料。然后开发出具有不同可生物降解特征的多孔丝绸样品。通过通过动物植入的细胞培养和体内评估进行体外评估，根据丝绸开发了出色的牙齿和骨骼再生材料。

项目成果

Characterization by Raman microspectroscopy of the strain-induced conformational transition in fibroin fibers from the silkworm Samia cynthia ricini

• DOI: 10.1021/bm060280w
• 发表时间: 2006-09-11
• 期刊: BIOMACROMOLECULES
• 影响因子: 6.2
• 作者: Rousseau, Marie-Eve;Beaulieu, Lilyane;Pezolet, Michel
• 通讯作者: Pezolet, Michel

Comparative study of silk fibroin porous scaffolds derived from salt/water and sucrose/hexafluoroisopropanol in cartilage formation

• DOI: 10.1016/j.jbiosc.2009.02.015
• 发表时间: 2009-07-01
• 期刊: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
• 影响因子: 2.8
• 作者: Makaya, Kumiko;Terada, Shinichi;Asakura, Tetsuo
• 通讯作者: Asakura, Tetsuo

Characterization of a Ca binding-amphipathic silk-like protein and peptide with the sequence (Glu)8(Ala-Gly-Ser-Gly-Ala-Gly)4 with potential for bone repair

• DOI: 10.1039/c1sm06646c
• 发表时间: 2012
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: Aya Nagano;H. Sato;Yumi Tanioka;Y. Nakazawa;D. Knight;T. Asakura

Biosynthesis and Characterization of the Artificial Protein Consisting of Marine Mussel Adhesive Protein and Silk-Like Protein Sequences

• DOI: 10.1295/polymj.pj2006148
• 发表时间: 2007-04
• 期刊: Polymer Journal
• 影响因子: 2.8
• 作者: Shinji Hatae;T. Asakura;Y. Fukushima

Small-diameter vascular grafts of Bombyx mori silk fibroin prepared by a combination of electrospinning and sponge coating

• DOI: 10.1016/j.matlet.2010.05.024
• 发表时间: 2010-08-31
• 期刊: MATERIALS LETTERS
• 影响因子: 3
• 作者: Sato, Michiko;Nakazawa, Yasumoto;Asakura, Tetsuo
• 通讯作者: Asakura, Tetsuo