Analysis of differential gene expression profiles induced by genotoxic and non-genotoxic carcinogens

基因毒性和非基因毒性致癌物诱导的差异基因表达谱分析

• 批准号: 18310047
• 负责人: FURIHATA Chie
• 金额: $ 4.19万
• 依托单位: Aoyama Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310047/
• 关键词: Toxicogenomics; gene expression profile; DNA microarray; quantitative real-time PCR; genotoxic; non-genotoxic; carcinogen; mouse liver; qPCR

The aim of this study is clarifying differential gene expression profiles and action mechanisms between genotoxic and non-genotoxic carcinogens in the target organ. The following results were obtained.1)Differential gene expression profiles of 2 N-nitroso carcinogenic compounds, diethylnitrosamine and dipropylnitrosamine, and a non-genotoxic carcinogenic compound, phenobarbital, in mouse liver at 4 h and 28 days after administration determined with DNA microarray and quantitative real-time PCR.2) Dose-dependent alteration in the gene expression in mouse liver induced by diethylnitrosamine and ethylnitrosourea at 4 h and 28 days after administration determined by quantitative real-time PCR.3) Time-dependent gene expression profiles at 4, 16, 20, 24, and 48 h after administration of a genotoxic carcinogen, chrysene, in mouse liver determined with quantitative real-time PCR.4) Differential gene expression profiles induced by 4 genotoxic carcinogens [quinoline, N-nitroso morpholine, 4-dimethyl (amino) azobenzene and 2, 4-diaminotoluene], and 2 non-genotoxic carcinogens (clofibrate and dichlorobenzene) in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as training set.5) Differential gene expression profiles induced by 4 genotoxic carcinogens [3, 4-(methylnitrosamino)-a-(3-pyridyl)-1-butanone, 2-actylamonofluorene, urethane, diisopropanolnitrosamine] and 3 non-genotoxic carcinogens [di(2-ethylhexyl)phthalate, dichloro-diphenyl-trichloroethane and furan] in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as validation set.

本研究的目的是阐明基因毒性和非基因毒性致癌物在靶器官中的差异基因表达谱和作用机制。得到了以下结果：1) 2种n-亚硝基致癌物二乙基亚硝胺和二丙基亚硝胺以及一种非遗传毒性致癌物苯巴比陀的差异基因表达谱；2)二乙基亚硝胺和乙基亚硝基脲在给药后4 h和28 d诱导小鼠肝脏基因表达量的剂量依赖性变化。3)基因毒性致癌物——甲苯给药后4、16、20、24和48 h的时间依赖性基因表达谱。4) 4种基因毒性致癌物[喹啉、n-亚硝基啉、4-二甲基（氨基）偶氮苯和2,4 -二氨基甲苯]和2种非基因毒性致癌物（氯贝特和二氯苯）在给药后4和48 h小鼠肝脏中诱导的差异基因表达谱，以实时荧光定量PCR检测为训练集。5) 4种基因毒性致癌物[3,4 -（甲基亚硝胺）-a-（3-吡啶基）-1-丁酮，2-乙酰氨基芴，聚氨酯，二异丙醇亚硝胺]和3种非基因毒性致癌物[二（2-乙基己基）邻苯二甲酸酯，二氯-二苯基-三氯乙烷和呋喃]在给药后4和48 h小鼠肝脏中诱导的差异基因表达谱，以实时荧光定量PCR检测为验证组。

项目成果

Collaborative study on the Toxicogenomics in JEMS/MMS II:High-throughput qPCR analysis by TagMan Low Density Array

JEMS/MMS II 毒物基因组学合作研究：TagMan 低密度阵列高通量 qPCR 分析

• 发表时间: 2007
• 作者: T Suzuki;T Koizumi;D Prabha;Y Luan;M Honma;S Hamada;M Nakajima;T Watanabe;C Furihata
• 通讯作者: C Furihata

Collaborative study of JEMS/MMS/ToXicogenomics:Quantitative real-time PCR analysis on mouse liver carcinogens

JEMS/MMS/ToXicogenomics合作研究：小鼠肝癌致癌物的实时定量PCR分析

• 发表时间: 2007
• 作者: Chie Furihata;Takashi Watanabe;Ayami Tadakuma;Mikiya Sakurai;Takayoshi Suzuki;Shuichi;Hamada;Kazunori Narumi;Madoka Nakajima;Akiko Koeda;Tomohiro Sakuma;Keiyu Oshida;Hisakazu Sanada;Michiasa Hirayama
• 通讯作者: Michiasa Hirayama

Differential gene expression induced by two N-nitroso carcinogens,phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR

寡核苷酸微阵列和实时定量PCR检测两种N-亚硝基致癌物苯巴比妥和乙醇诱导的小鼠肝脏差异基因表达

• 发表时间: 2007
• 期刊: Genes and Environment 29
• 作者: Takashi Watanabe;Kaori Tobe;Yutaka Nakachi;Yasumitsu Kondoh;Madoka Nakajima;Shuichi Hamada;Chiaki Namiki;Takayoshi Suzuki;Satoru Maeda;Ayami Tadakuma;Mikiya Sakurai;Yuko Arai;Hyogo Atsushi;Masako Hoshino;Tomoko Tashiro;Hisashi Ito;Hiroshig
• 通讯作者: Hiroshig

Collaborative study on the Toxicogenomics in JEMS/MMS II : High-throughput qPCR analysis by TaqMan Low Density Array

JEMS/MMS II 毒物基因组学合作研究：通过 TaqMan 低密度阵列进行高通量 qPCR 分析

• 发表时间: 2007
• 作者: T;Suzuki;T;Koizumi;D;Prabha;Y;Luan;M;Honma;S;Hamada;M;Nakajima;T;Watanabe;C;Furihata
• 通讯作者: Furihata

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄