Characterization of stress responses and its application to the lifespan regulation in model microorganisms.

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To understand the strategy of stress responses of microorganisms, I characterized two model microorganisms, E.coli and S.pombe. In E.coli, I found that a response regulator, ArcA is involved in fragella synthesis in addition to the well known aerobic-anaerobic responses. I also identified the whole genome binding sites of transcription factors, ArcA, H-NS and Fnr of E. coli by using ChIP-CHIP analysis.By using fission yeast, S pombe, I started to identify and characterize factors involved in the chronological lifespan. In this project, I identified novel factors, Ecll family proteins that increase chronological lifespan of S. pombe when overexpressed. I also isolated some mutants and clones that affect chronological lifespan of S pombe. Based on these results, I was able to establish the basis for the development of the future research.