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Enhancement of sink activity by controling the gene expression and the activity of invertase and sorbitol dehydrogenase

通过控制基因表达以及蔗糖酶和山梨糖醇脱氢酶的活性增强库活性

基本信息

批准号：
18380021
负责人：
YAMAKI Shohei
金额：
$ 10.55万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380021/
关键词：
rose petal FaSDH strawberry invertase Japanese pear PsV-AIV1-2 sorbitol dehydrogenase splicing バラ花弁 Ps-AIV1 Ps-AIV2

项目摘要

The role of acid invertase for cell enlargement and its regulation of gene expression were investigated using the systems of fruit enlargement of Japanese pear and of petal enlargement of rose. (1) Two full length vacuolar type acid invertase cDNA (PsV-AIV1, PsV-AIV2) were cloned from Japanese pear fruit. PsV-AIV1 related the early growth concerning active cell division and PsV-AIV2 related mainly cell enlargement with sugar accumulation. (2) The enlargement of rose petal was initiated by forming high turgor pressure which was formed by converting sucrose to hexose via the rising of invetase activity with increase of gene expression. The rising of activity was induced by auxin treatment.The regulation of NAD-SDH gene expression and the accumulation of sorbitol were investigated using straw berry fruit. (1) The full length cDNA of NAD-SDH of strawberry fruit (FaSDH) was cloned and the activity also detected. However, relating sorbitol-6-phosphate dehydrogenase the gene existed but the protein and activity were not detected. The kinetics of NAD-SDH activity revealed that sorbitol was synthesized by the reduced reaction of fructose. (2) NAD-SDH activity was stimulated by fructose, sorbitol or auxin treatment. However, there were not clear changes of mRNAs by them. (3) The mRNA of NAD-SDH were composed of the pre-mature mRNA containing intron and the mature mRNA. The stimulation of NAD-SDH activity by fructose, sorbitol or auxin depended on the stimulation of splicing rate after transcription.As described above, it was indicated that acid invertase and sorbitol dehydrogenase play an important role for the cellular enlargement and sugar accumulation and their activities were regluated by the substrates and phytohormone.

以日本梨果实膨大系统和月季花瓣膨大系统为材料，研究了酸性转化酶在细胞扩大中的作用及其对基因表达的调控。(1)从日本梨果实中克隆了两个全长的液泡型酸性转化酶基因(PSV-AIV1，PSV-AIV2)。PSV-AIV1与细胞分裂活跃有关，PSV-AIV2主要与细胞增大和糖分积累有关。(2)玫瑰花瓣的膨大是由高膨压启动的，高膨压是通过内切酶活性的升高和基因表达的增加将蔗糖转化为己糖而形成的。以草莓果实为试材，研究了NAD-SDH基因表达的调控和山梨醇的积累。(1)克隆了草莓果实NAD-SDH全长基因(FaSDH)，并对其活性进行了检测。然而，与山梨醇-6-磷酸脱氢酶相关的基因存在，但没有检测到其蛋白质和活性。NAD-SDH活性的动力学研究表明，山梨醇是由果糖还原合成的。(2)果糖、山梨醇或生长素对NAD-SDH活性有促进作用。然而，它们对mRNAs的影响不明显。(3)NAD-SDH基因由含有内含子的早熟基因和成熟基因组成。果糖、山梨醇或生长素对NAD-SDH活性的刺激依赖于转录后剪接率的刺激。上述结果表明，酸性转化酶和山梨醇脱氢酶在细胞放大和糖积累中起重要作用，它们的活性受到底物和植物激素的调节。