Study for lymphangiogenesis and tumor immunity by artificial lymphoid tissue construction

将基质细胞包埋于胶原支架中，可构建组织工程人工淋巴组织（aLT）。在这项研究中，我们证实了aLTs的功能性传出淋巴管形成。结果表明，alt具有不同于普通血管或高内皮小静脉的特化血管。此外，我们发现活化的淋巴细胞集中在alt中，这使得alt能够在严重联合免疫缺陷小鼠中引入有效的细胞毒性T细胞活性。我们的数据表明，alt可能用于治疗难治性传染病或癌症的适应性免疫激活。

A tissue-engineered artificial lymphoid tissue (aLT) can be constructed by introducing stromal cells embedded in a collagenous scaffold. In this study, we confirmed functional efferent lymphatic vessel formation in aLTs. It was revealed aLTs had specialized blood vessels distinct from ordinary blood vessels or high endothelial venules. Further, we found that activated lymphocytes were concentrated in aLTs, which enabled aLTs to introduce effective cytotoxic T cell activities in severe-combined immunodeficient mice. Our data suggested that possible therapeutic application of aLTs for adaptive immunity activation against intractable infectious diseases or cancer.