Proteomic Analysis of Psychological Stress and Protein Higher Structures

心理压力与蛋白质高级结构的蛋白质组学分析

• 批准号: 18390211
• 负责人: KAWAMURA Noriyuki
• 金额: $ 11.19万
• 依托单位: National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390211/
• 关键词: Proteomics; Higher Structure; Stress; プロテオーム解析; アルブミン; 酸化

We hypothesized that the psychological stress affects physical functions through alterations in disulfide bondage formation and free cysteine modifications.We have shown that the cysteine residue, 34^<th> amino acid, of Albumin is oxidated by psychological stress consequences and this change evokes Albumin higher structure alteration that leads the functional decrementation into half and alternative fragmentation of Albumin. This characteristic change is observed in the plasma samples of the patients for Major Depressive Disorder.Thus, we searched the degradome (fragments from albumin) and or peptidome (Albumin related peptides) within the plasma of MDD patients, using the LCMS procedures.We have found 270 peptides which solely existed in the plasma of MDD and wrote patent document in Japan. The contents will be hidden until it is opened.On the other hand, we made a research collaboration relationship with Protosera Company and Human Metabolome Technologies The former can evaluate the quantity of peptidome briefly by MALDI-MS Blot-Chip system, and the latter can measure the metabolome by CE-MS.The other patent document was also written already.The details cannot be written here now.

我们假设心理压力通过改变二硫键的束缚形成和自由半胱氨酸的修饰会影响身体功能。我们表明，白蛋白的半胱氨酸残基34^<th氨基酸被心理压力后果氧化，并被心理压力后果氧化，而这种变化的较高的白蛋白变化使白蛋白的变化导致功能降低到一半和蛋白蛋白白蛋白的功能降低。 This characteristic change is observed in the plasma samples of the patients for Major Depressive Disorder.Thus, we searched the degradome (fragments from albumin) and or peptidome (Albumin related peptides) within the plasma of MDD patients, using the LCMS procedures.We have found 270 peptides which solely existed in the plasma of MDD and wrote patent document in Japan.这些内容将被隐藏在打开之前。另一方面，我们与Protosera Company建立了研究合作关系，而人类代谢组技术可以通过Maldi-MS Blot-Chip系统简要评估肽组的数量，而后者也可以通过CE-MS。还可以撰写的其他详细信息。

项目成果

MTsystem discussion for Medical Sciences

医学科学 MTsystem 讨论

• 发表时间: 2008
• 期刊: Journal of Quality Control vol16. No.1
• 作者: 埴淵知哉;平井寛;近藤克則;前田小百合;相田潤;市田行信;N Kawamura
• 通讯作者: N Kawamura

Trauma intensity and posttraumatic stress :implications of the tsunami experience in Sri Lanka for the management of future disasters, Psychosomatic Medicine, Intemational Congress Series 1287,

创伤强度和创伤后应激：斯里兰卡海啸经历对未来灾害管理的影响，心身医学，国际大会系列 1287，

• 发表时间: 2007
• 作者: Dewaraja R;Kawamura N
• 通讯作者: Kawamura N

Proteomic analysis of the psychiatric disorders and protein biomaker for psychological stress.

精神疾病的蛋白质组学分析和心理压力的蛋白质生物制造商。

• 发表时间: 2006
• 期刊: Psychosomatic Medicine International Congress Series 1287
• 作者: N.Kawamura;R.Dewaraja;Y.Ishii;N.Mendis;N.Mendis:
• 通讯作者: N.Mendis:

Trauma intensity and posttraumatic stress : implications of the tsunami experience in Sri Lanka for the management of future disasters.

创伤强度和创伤后应激：斯里兰卡海啸经历对未来灾害管理的影响。

• 发表时间: 2007
• 期刊: Psychosomatic Medicine, International Congress Series 1287, ELSEVIER, Netherlands 1287
• 作者: Dewaraja R;Kawamura N :
• 通讯作者: Kawamura N :

Lazer Therapy for Autunomic Nervous System Disregulation.

激光疗法治疗自主神经系统失调。

• 发表时间: 2007
• 作者: Hirofumi Iimori;Noriyuki Kawamura.
• 通讯作者: Noriyuki Kawamura.