Proteomic Analysis of Psychological Stress and Protein Higher Structures

心理压力与蛋白质高级结构的蛋白质组学分析

• 批准号: 18390211
• 负责人: KAWAMURA Noriyuki
• 金额: $ 11.19万
• 依托单位: National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390211/
• 关键词: Proteomics; Higher Structure; Stress; プロテオーム解析; アルブミン; 酸化

We hypothesized that the psychological stress affects physical functions through alterations in disulfide bondage formation and free cysteine modifications.We have shown that the cysteine residue, 34^<th> amino acid, of Albumin is oxidated by psychological stress consequences and this change evokes Albumin higher structure alteration that leads the functional decrementation into half and alternative fragmentation of Albumin. This characteristic change is observed in the plasma samples of the patients for Major Depressive Disorder.Thus, we searched the degradome (fragments from albumin) and or peptidome (Albumin related peptides) within the plasma of MDD patients, using the LCMS procedures.We have found 270 peptides which solely existed in the plasma of MDD and wrote patent document in Japan. The contents will be hidden until it is opened.On the other hand, we made a research collaboration relationship with Protosera Company and Human Metabolome Technologies The former can evaluate the quantity of peptidome briefly by MALDI-MS Blot-Chip system, and the latter can measure the metabolome by CE-MS.The other patent document was also written already.The details cannot be written here now.

我们假设心理应激通过改变二硫键的形成和游离半胱氨酸的修饰来影响生理功能，我们已经表明，白蛋白的半胱氨酸残基，34^<th>氨基酸，被心理应激的后果氧化，这种变化引起白蛋白更高的结构改变，导致白蛋白的功能减少到一半和选择性片段化。因此，我们使用LCMS程序搜索MDD患者血浆中的降解组（来自白蛋白的片段）和/或肽组（白蛋白相关肽），我们发现了270种肽，这些肽仅存在于MDD患者的血浆中，并在日本撰写了专利文件。另一方面，我们与Protosera公司和Human Metabolome Technologies公司建立了研究合作关系，前者可以通过MALDI-MS Blot-Chip系统简单评估肽组的数量，后者可以通过CE-MS测量代谢组。另一个专利文件也已经写好了，具体内容在这里不能写。

项目成果

MTsystem discussion for Medical Sciences

医学科学 MTsystem 讨论

• 发表时间: 2008
• 期刊: Journal of Quality Control vol16. No.1
• 作者: 埴淵知哉;平井寛;近藤克則;前田小百合;相田潤;市田行信;N Kawamura
• 通讯作者: N Kawamura

Proteomic analysis of the psychiatric disorders and protein biomaker for psychological stress.

精神疾病的蛋白质组学分析和心理压力的蛋白质生物制造商。

• 发表时间: 2006
• 期刊: Psychosomatic Medicine International Congress Series 1287
• 作者: N.Kawamura;R.Dewaraja;Y.Ishii;N.Mendis;N.Mendis:
• 通讯作者: N.Mendis:

Trauma intensity and posttraumatic stress :implications of the tsunami experience in Sri Lanka for the management of future disasters, Psychosomatic Medicine, Intemational Congress Series 1287,

创伤强度和创伤后应激：斯里兰卡海啸经历对未来灾害管理的影响，心身医学，国际大会系列 1287，

• 发表时间: 2007
• 作者: Dewaraja R;Kawamura N
• 通讯作者: Kawamura N

Trauma intensity and posttraumatic stress : implications of the tsunami experience in Sri Lanka for the management of future disasters.

创伤强度和创伤后应激：斯里兰卡海啸经历对未来灾害管理的影响。

• 发表时间: 2007
• 期刊: Psychosomatic Medicine, International Congress Series 1287, ELSEVIER, Netherlands 1287
• 作者: Dewaraja R;Kawamura N :
• 通讯作者: Kawamura N :

Lazer Therapy for Autunomic Nervous System Disregulation.

激光疗法治疗自主神经系统失调。

• 发表时间: 2007
• 作者: Hirofumi Iimori;Noriyuki Kawamura.
• 通讯作者: Noriyuki Kawamura.