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Basic study of fetal heart ischemia by using a multi-electrodes fetal electrocardiogram via maternal abdomen for all gestational period fetuses

经母腹多电极胎儿心电图对各孕期胎儿进行胎儿心脏缺血的基础研究

基本信息

批准号：
18390306
负责人：
KIMURA Yoshitaka
金额：
$ 8.84万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Methods: Experiments were performed with pregnant C57BL/6N mice and 17 dpc fetuses. The measurements were carried out in the Fluid Dynamics Laboratory of the Tohoku University to take advantage of their ultrasonogram experimental facilities for animals. Caesarean section was carried out in pregnant mice after anesthesia and electrodes were attached to the fetuses to measure fetal ultrasonogram. 5 minute clipping and 5 minute release of the branches of the uterine artery was performed three times in tandem and the results were monitored by ultrasonogram.Results: The fetal heart potential was as low as a few microvolts (μV) and because of this low signal extraction of the fetal electrocardiogram proved to be very troublesome. To overcome this problem, we developed a needle electrode that contained a signal amplification mechanism and were able to reduce the noise to a minimal level. To our knowledge, we are the first group in the world to report the successful measurement of the fetal el … More ectrocardiogram. Moreover, based on these investigations, we were able to identify the characteristic "T-wave" from the fetal electrocardiogram that had been so far believed to be nonexistent in mice. Next, we investigated the alterations in the electrocardiogram pattern and fetal heart rate because of the periodic constraint and release (5 minutes each, 3 times repeated) of the branches of the uterine artery. The results showed increase of the T-wave during constraint by clipping of the branches of the artery, accompanied with frequent arrhythmia, which lead to a reduction of fetal heart rate. After release of the clipping, frequent arrhythmia was again observed and the heart rate gradually improved to a normal level. Furthermore, during these analyses we were able to extract an ultrasonogram from the fetal brain that showed the blood flow in the branches of the middle cerebral artery. However, due to the breathing of the mother, it was difficult to obtain a clear image from the ultrasonogram that showed the area of interest in the brain. Because of this hindrance, we were unable to produce a real-time image of the blood flow in the brain during constraint and release of the branches of the uterine artery.Discussion: Our analysis shows that fetal electrocardiogram and ultrasonogram can be a useful tool to monitor fetal ischemia. Less

方法：用妊娠C57 BL/6 N小鼠和17 dpc胎鼠进行实验。测量是在东北大学流体力学实验室利用其动物超声波实验设备进行的。孕鼠麻醉后行剖腹产，胎鼠接上电极，测量胎鼠声像图。结果：胎儿心脏电位低至几微伏（μV），胎儿心电图信号的提取非常困难。为了克服这个问题，我们开发了一种包含信号放大机制的针电极，并且能够将噪声降低到最低水平。据我们所知，我们是世界上第一个报告成功测量胎儿el的小组。 ...更多信息心电图此外，基于这些研究，我们能够从胎儿心电图中识别出迄今为止被认为在小鼠中不存在的特征性“T波”。接下来，我们研究了由于子宫动脉分支的周期性限制和释放（每次5分钟，重复3次）而引起的心电图模式和胎心率的变化。结果显示，胎儿在动脉分支夹闭时T波增高，并伴有频繁心律失常，导致胎心率减慢。释放夹闭后，再次观察到频繁心律失常，心率逐渐改善至正常水平。此外，在这些分析过程中，我们能够从胎儿大脑中提取超声图像，显示大脑中动脉分支的血流。然而，由于母亲的呼吸，很难从超声波图中获得清晰的图像，显示大脑中的感兴趣区域。由于这种阻碍，我们无法产生一个实时图像的血流在大脑中的限制和释放的分支的子宫artery.Discussion：我们的分析表明，胎儿心电图和超声波可以是一个有用的工具来监测胎儿缺血。少