Craniofecial bone reganeration by mesenchymal stem cells of the craniofacial origin

人骨髓间充质干细胞、hMSCs在颅面部区域进行移植后动力学测试，转染人绿色荧光蛋白（hGFP）CDNA，然后研究细胞归巢和分化。对hGFP转染的hMSC进行1至4代体外比较测试。跟踪细胞转染效率，第 1 代中的 3 小时和 6 小时转染与前 3 天的未转染细胞相似。这些细胞用于辐射后、颅面和头颈相关的组织再生。瘢痕疙瘩临床系列也针对人类间充质干细胞进行了测试。在体内照射后，间充质干细胞有显着的效率。此外，hMSC 还被证明与纤维增殖性疾病 FPD 相关。这些细胞可作为储备用于紧急放射疾病治疗，因为这些细胞被冷冻并且解冻后 hGFP 标记保持一致。人类间充质干细胞现在在西方国家被用作细胞库细胞。因此，日本也应该考虑向这个方向延伸细胞。 hGFP标记的hMSCs在血流量减少和受损神经以及颅骨缺损模型的时间和空间上均得到显着恢复，并在体内进行了测试。因此，应计划使用自体组织干细胞进行进一步的临床前和临床测试。

Human bone marrow derived mesenchymal stem cell, hMSCs were tested for post-grafting kinetics in the craniofacial regions, human Green Fluorescent Protein (hGFP)CDNA was trasfected and then the cell-homing and differentiation were investigated. The hGFP-transfected hMSCs were comparatively tested from 1 to 4 passages in vitro. The efficiency of the cell-transfection was followed and 3-hour and 6-hour transfections in the 1 passage were similar to non-transfected cells for the first 3 days. These cells were used for post-irradiated, craniofacial-, and head and neck-related tissue regeneration. And the keloid clinical series were also tested in terms of human mesenchymal stem cells. In the post-irradiation in vivo, there was remarkable efficiency of mesenchymal stem cells. Also, hMSCs demonstrated to the relationship to the fibroproliferative disease, FPD. These cells may be used for emergency radiation diseases as stockpiles since these cells are frozen and hGFP-labeling are consistent after thawing. The human mesenchymal stem cells are now used for cell-bank cells in the Western countries. Thus, the extensions of the cells in this direction should be considered in Japan as well. hGFP-labeled hMSCs are in vivo tested in the decreased blood flows and impaired nerves demonstrated the significant recovery as well as the cranial bone-defect models temporally and spaciously. Therefore, further preclinical and clinical tests should be planned with using autologous tissue-derived stem cells.