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Craniofecial bone reganeration by mesenchymal stem cells of the craniofacial origin

颅面源间充质干细胞的颅面骨再生

基本信息

批准号：
18390478
负责人：
HIRANO Akiyoshi
金额：
$ 10.54万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390478/
关键词：
transfection human bone marrow-derived mesenchvmal stem cells tissue regeneration and healing congenital anomaly life-style oriented diseases bone maturation marker vessel and nerve regeneration facial bone growth 頭蓋顔面ヒト間葉系幹細胞頭蓋顔面骨 /

项目摘要

Human bone marrow derived mesenchymal stem cell, hMSCs were tested for post-grafting kinetics in the craniofacial regions, human Green Fluorescent Protein (hGFP)CDNA was trasfected and then the cell-homing and differentiation were investigated. The hGFP-transfected hMSCs were comparatively tested from 1 to 4 passages in vitro. The efficiency of the cell-transfection was followed and 3-hour and 6-hour transfections in the 1 passage were similar to non-transfected cells for the first 3 days. These cells were used for post-irradiated, craniofacial-, and head and neck-related tissue regeneration. And the keloid clinical series were also tested in terms of human mesenchymal stem cells. In the post-irradiation in vivo, there was remarkable efficiency of mesenchymal stem cells. Also, hMSCs demonstrated to the relationship to the fibroproliferative disease, FPD. These cells may be used for emergency radiation diseases as stockpiles since these cells are frozen and hGFP-labeling are consistent after thawing. The human mesenchymal stem cells are now used for cell-bank cells in the Western countries. Thus, the extensions of the cells in this direction should be considered in Japan as well. hGFP-labeled hMSCs are in vivo tested in the decreased blood flows and impaired nerves demonstrated the significant recovery as well as the cranial bone-defect models temporally and spaciously. Therefore, further preclinical and clinical tests should be planned with using autologous tissue-derived stem cells.

检测人骨髓间充质干细胞（hMSCs）在颅面部移植后的动力学，转染人绿色荧光蛋白（hGFP）cDNA，观察hMSCs在颅面部的归巢和分化情况。将hGFP转染的hMSCs在体外进行1 ~ 4代的比较检测。跟踪细胞转染的效率，并且在第1代中的3小时和6小时转染与前3天的未转染细胞相似。这些细胞被用于照射后，颅面，头部和颈部相关的组织再生。瘢痕疙瘩的临床系列也进行了测试方面的人骨髓间充质干细胞。在体内照射后，骨髓间充质干细胞有显著的效率。hMSCs与纤维增生性疾病（FPD）的关系也得到证实。这些细胞可以作为储备用于紧急放射性疾病，因为这些细胞是冷冻的，并且解冻后hGFP标记是一致的。目前，在西方国家，人骨髓间充质干细胞被用作细胞库细胞。因此，在日本也应该考虑在这个方向上扩大细胞。hGFP标记的hMSCs在血流量减少和受损神经中的体内测试显示了显著的恢复以及颅骨缺损模型的时间和空间。因此，应计划使用自体组织来源的干细胞进行进一步的临床前和临床试验。