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Biological effect of Polarized Light Emitting Diode Irradiation on cell body

偏振发光二极管照射对细胞体的生物学效应

基本信息

批准号：
18591624
负责人：
IKEDA Kazuo
金额：
$ 2.52万
依托单位：
Department of Clinical Research, National Hospital Organization Kanazawa Medical Center (2007)Kanazawa University (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

BackgroundI would like to propose a new phototherapy using polarized light from Light Emitting Diode (LED). The purpose of this study is to clarify the effect of polarized LED irradiation on wound healing. MethodsFive groups were classified as control (C), unpolarized (U), linearly polarized (L), right circularly polarized (RC), and left circularly polarized (LC) LED irradiation. In vitro study, fibroblast cell cultures were irradiated, and cellular proliferation was evaluated with a WST-8 assay. In vivo study, full-thickness skin defect of 20mm diameter was created on the dorsal side of rats. The ratio of the residual wound area was measured and expression of type 1 and type 3 procollagen mRNA in granulation tissue was determined by real-time RT-PCR method.Results The cellular proliferation rates of group RC and L were significantly higher than other groups. The ratio of the residual wound area of group RC and L were significantly reduced than group C and U. Expression of type1procollagen mRNA in group RC was significantly increased about 1.5-fold in comparison to the group C. There were no significant differences for about type 3 procollagen.ConclusionsThe right circularly polarized light and linearly polarized light promoted the process of wound healing by increasing the proliferation of fibroblasts, and the right circularly polarized light increased the expression of type 1 procollagen mRNA. The effectiveness of right circularly polarized light suggests that some optical active material which has a circular dichroic spectrum takes part in a biochemical reaction.

背景我想提出一种使用发光二极管（LED）偏振光的新光疗。本研究的目的是阐明偏振 LED 照射对伤口愈合的影响。方法将五组分为对照（C）、非偏振（U）、线偏振（L）、右圆偏振（RC）和左圆偏振（LC）LED照射。在体外研究中，对成纤维细胞培养物进行辐照，并使用 WST-8 测定法评估细胞增殖。体内研究中，在大鼠背侧制作直径20mm的全层皮肤缺损。测定残余创面面积比例，实时RT-PCR法测定肉芽组织中1型和3型前胶原mRNA表达量。结果RC组和L组细胞增殖率显着高于其他组。 RC、L组创面残留面积比例较C、U组明显减少。RC组1型原胶原mRNA表达较C组显着增加约1.5倍，3型原胶原mRNA表达量无显着性差异。圆偏振光增加了 1 型前胶原 mRNA 的表达。右旋圆偏振光的有效性表明，一些具有圆二向色光谱的光学活性材料参与了生化反应。