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Biological effect of Polarized Light Emitting Diode Irradiation on cell body

偏振发光二极管照射对细胞体的生物学效应

基本信息

批准号：
18591624
负责人：
IKEDA Kazuo
金额：
$ 2.52万
依托单位：
Department of Clinical Research, National Hospital Organization Kanazawa Medical Center (2007)Kanazawa University (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

BackgroundI would like to propose a new phototherapy using polarized light from Light Emitting Diode (LED). The purpose of this study is to clarify the effect of polarized LED irradiation on wound healing. MethodsFive groups were classified as control (C), unpolarized (U), linearly polarized (L), right circularly polarized (RC), and left circularly polarized (LC) LED irradiation. In vitro study, fibroblast cell cultures were irradiated, and cellular proliferation was evaluated with a WST-8 assay. In vivo study, full-thickness skin defect of 20mm diameter was created on the dorsal side of rats. The ratio of the residual wound area was measured and expression of type 1 and type 3 procollagen mRNA in granulation tissue was determined by real-time RT-PCR method.Results The cellular proliferation rates of group RC and L were significantly higher than other groups. The ratio of the residual wound area of group RC and L were significantly reduced than group C and U. Expression of type1procollagen mRNA in group RC was significantly increased about 1.5-fold in comparison to the group C. There were no significant differences for about type 3 procollagen.ConclusionsThe right circularly polarized light and linearly polarized light promoted the process of wound healing by increasing the proliferation of fibroblasts, and the right circularly polarized light increased the expression of type 1 procollagen mRNA. The effectiveness of right circularly polarized light suggests that some optical active material which has a circular dichroic spectrum takes part in a biochemical reaction.

背景我想提出一种新的光疗，使用来自发光二极管（LED）的偏振光。本研究的目的是阐明偏振LED照射对伤口愈合的影响。方法分为对照组（C组）、非偏振组（U组）、线偏振组（L组）、右旋偏振组（RC组）和左旋偏振组（LC组）。在体外研究中，对成纤维细胞培养物进行辐照，并采用WST-8试验评价细胞增殖。在体实验中，在大鼠背部造成直径为20 mm的全层皮肤缺损。结果RC组和L组创面肉芽组织中1型和3型前胶原mRNA的表达明显高于其他各组，差异有统计学意义（P < 0. 05）。RC组和L组的残余创面面积比例明显低于C组和U组。RC组1型前胶原mRNA的表达较C组显著增加约1.5倍。结论右旋偏振光和线偏振光通过促进成纤维细胞增殖促进创面愈合，右旋偏振光可促进1型前胶原mRNA的表达。右旋圆偏振光的有效性表明，某些具有圆二色性光谱的光学活性物质参与了生化反应。