Biological effect of Polarized Light Emitting Diode Irradiation on cell body

我想提出一种新的利用发光二极管（LED）偏振光的光疗方法。本研究的目的是阐明偏振LED照射对伤口愈合的影响。方法5组分别为对照组(C)、非偏振组(U)、线偏振组(L)、右圆偏振组（RC）和左圆偏振组（LC）。在体外研究中，对成纤维细胞培养物进行辐照，用WST-8法评价细胞增殖。在体内实验中，在大鼠背部制造直径为20mm的全层皮肤缺损。采用实时荧光定量pcr法测定肉芽组织中1型和3型前胶原mRNA的表达。结果RC组和L组细胞增殖率显著高于其他各组。RC组和L组残创面面积比C组和u组显著降低，1型前胶原mRNA表达量较C组显著升高约1.5倍，3型前胶原mRNA表达量无显著差异。结论右圆偏振光和线偏振光通过增加成纤维细胞的增殖促进创面愈合，右圆偏振光增加1型前胶原mRNA的表达。右圆偏振光的有效性表明，某些具有圆二向色光谱的光学活性物质参与了生化反应。

BackgroundI would like to propose a new phototherapy using polarized light from Light Emitting Diode (LED). The purpose of this study is to clarify the effect of polarized LED irradiation on wound healing. MethodsFive groups were classified as control (C), unpolarized (U), linearly polarized (L), right circularly polarized (RC), and left circularly polarized (LC) LED irradiation. In vitro study, fibroblast cell cultures were irradiated, and cellular proliferation was evaluated with a WST-8 assay. In vivo study, full-thickness skin defect of 20mm diameter was created on the dorsal side of rats. The ratio of the residual wound area was measured and expression of type 1 and type 3 procollagen mRNA in granulation tissue was determined by real-time RT-PCR method.Results The cellular proliferation rates of group RC and L were significantly higher than other groups. The ratio of the residual wound area of group RC and L were significantly reduced than group C and U. Expression of type1procollagen mRNA in group RC was significantly increased about 1.5-fold in comparison to the group C. There were no significant differences for about type 3 procollagen.ConclusionsThe right circularly polarized light and linearly polarized light promoted the process of wound healing by increasing the proliferation of fibroblasts, and the right circularly polarized light increased the expression of type 1 procollagen mRNA. The effectiveness of right circularly polarized light suggests that some optical active material which has a circular dichroic spectrum takes part in a biochemical reaction.