Usefulness of a novel hydroxyapatite fiber as a scaffold of stem cells for bone regeneration in spinal fusion surgery

新型羟基磷灰石纤维作为干细胞支架在脊柱融合手术中用于骨再生的用途

• 批准号: 18591642
• 负责人: MATSUMOTO Morio
• 金额: $ 1.62万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591642/
• 关键词: tissue engineering; clinical situation; bone arafting; spine fusion surgery; 移植、再生医療; 移植・再生医療

Spinal fusion was conducted in rats using apatite fiber mesh (AFM) loaded with rhBMP-2. The percentage of fusion rate was 100% and concluded that AFM might be effective as a bone substitute in spinal fusion operation. However, as AFM was fragile, we needed to operate carefully on handling. In order to enhance the mechanical properties, we have developed chelete-setting apatite cement using hydroxyapatite particles surfaced-modified with inositol phosphate (IP_6-HAp). The aim of this study is to develop IP_6-HAp as a material for bone grafting. In 2006 and 2007, we have done some studies listed below.1. MC3T3-E1 cells were seeded and cultured on IP_6-HAp. After cell culture, the cell number attached on them was counted to examine initial cell-attachment efficiency and cell proliferation.2. IP_6-HAps were implanted into tibiae of rabbits, together with a control of Biopex[○!R]-R (PENTAX Corporation, Japan). Within 24 weeks after implantation, the rabbits were sacrificed to retrieve the specimens with the surrounding bone for histological evaluation.In experiment 1, The MC3T3-E1 cells cultured on IP_6-HAp showed lower cell attachment and cell proliferation than the control (polystyrene 12-wells plate);. However, cell morphology observed by SEM showed that cells on IP_6-Hap nearly attained to be confluent for 7 days. These results demonstrate that IP_6-Hap have noncytotoxicity.In experiment 2, mature bone was seen to have formed around the surface of the IP_6-HAps as compared with Biopex[○!R]-R. The newly-formed bone was in close contact with the cement surface, without any sign of intervening fibrous tissue layer. This result indicates that IP_6-HAp has an excellent osteoconductivity to living bone.These results have shown that IP_6-HAp has noncytotoxicity. Furthermore, the hard tissue response of IP_6-HAp is shown to be similar to that of Biopex[○!R]-R. Thus, we conclude that IP_6-HAp is useful as a material for novel bone grafting.

使用负载rhBMP-2的磷灰石纤维网（AFM）在大鼠中进行脊柱融合。融合率为100%，表明AFM可作为脊柱融合术的骨替代物。然而，由于AFM是脆弱的，我们需要小心操作的处理。为了提高磷灰石水泥的力学性能，我们用磷酸肌醇对羟基磷灰石颗粒进行表面改性（IP_6-HAp）。本研究的目的是研制IP_6-HAp骨移植材料。在2006年和2007年，我们做了一些研究，如下所列。将MC 3 T3-E1细胞接种于IP_6-HAp上培养。细胞培养后，计数贴壁细胞数，检测细胞初始贴壁率和细胞增殖情况.将IP_6-HAps植入家兔胫骨，并与Biopex[○！R]-R（PENTAX Corporation，日本）。实验1：MC 3 T3-E1细胞在IP_6-HAp上的贴壁和增殖能力均低于对照组（聚苯乙烯12孔威尔斯板）;扫描电镜观察细胞形态显示，IP_6-Hap上的细胞在培养7天后基本达到融合状态。结果表明，IP_6-Hap无细胞毒性，与Biopex[○！R]-R。新生骨与骨水泥表面紧密接触，无任何纤维组织层介入迹象。结果表明，IP_6-HAp对活骨具有良好的骨传导性，无细胞毒性。此外，IP_6-HAp的硬组织反应与Biopex[○！R]-R。因此，我们认为IP_6-HAp是一种有用的新型骨移植材料。

项目成果

Fabrication of Chelate-setting cements from apatite powder synthesized via a wet process and its mechanical property

湿法合成磷灰石粉螯合水泥的制备及其力学性能

• 发表时间: 2007
• 期刊: Archives of BioCeramics Research 7
• 作者: Y.;Horiguchi;K.;Oribe;M.;Aizawa
• 通讯作者: Aizawa

Fabrication of Chelate-setting cements from apatite powder synthesizedvia a wet process and its mechanical property

湿法合成磷灰石粉螯合水泥的制备及其力学性能

• 发表时间: 2007
• 期刊: Archives of BioCeramics Research 7
• 作者: Y. Horiguchi;K. Oribe;M. Aizawa
• 通讯作者: M. Aizawa

Surgical strategy for cervical spondylotic myelopathy based on retrospec tive long-term follow-up study

基于回顾性长期随访研究的脊髓型颈椎病手术策略

• 发表时间: 2007
• 期刊: J Jpn Soc Spine Surg related Res 17(3)
• 作者: Chiba K;Ogawa Y;Ishii K;Takaishi H;Nakamura M;Matsumoto M;et. al.
• 通讯作者: et. al.

アパタイトファイバーにより創製した三次元scaffoldsによめる硬組織の再生

使用由磷灰石纤维制成的三维支架再生硬组织

• 发表时间: 2008
• 作者: 森末 光;松本 守雄;千葉 一裕;戸山 芳昭;相澤 守;ほか
• 通讯作者: ほか

Fabrication of Biodegradable β-tricalcium Phosphate/poly-(L-Lacticacid)Hybrids and Their In Vitro Evaluation

可生物降解的β-磷酸三钙/聚(L-乳酸)杂化物的制备及其体外评价

• 发表时间: 2007
• 期刊: Archives of BioCeramics Research 7
• 作者: Y. Iwamoto;T. Miyashita;Y. Tanaka;N. Sugiyama;Y. Takeoka;M. Rikukawa;M. Aizawa
• 通讯作者: M. Aizawa