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Effect of abnormal body temperature on ventilator induced lung injury and acute phase reaction

体温异常对呼吸机所致肺损伤及急性时相反应的影响

基本信息

批准号：
18591710
负责人：
NOGUCHI Takayuki
金额：
$ 1.92万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591710/
关键词：
acute lung injury hyperthe mia hypothermia inflamation cytokine ハイポサーミアハイパーサーミア NF-kB IL-1β IL-6 TNF-α HSP HSF

项目摘要

To examine whether moderate changes in cell culture temperature influence the production of various cytokines and associated mediators of inflammation. We performed lipopolysaccharide (LPS) stimulation of the murine macrophagic RAW264.7 cell line under hyperthermic (40℃), normothermic (37℃) and hypothermic (34℃) conditions. We then measured the levels of heat shock protein 70 (HSP70), heat shock factor protein (HSF) and nuclear factor-kB (NF-kB) dimers (p50 and p65)in the cells, and the levels of high mobility group box 1 (HMGB1) and the cytokines tumor necrosis fector-oc (TNF-a), interleukin ip (IL-ip) and interleukin 6 (IL-6) n the culture supernatants. Levels of HMGB1, IL-ip, IL-6,and TNF-a, as well as NF-kB dimers (p50 and p65), were all reduced following LPS stimulation at 40℃ and 34℃ compared with those at 37℃. Levels of HSP70 and HSF increased at 40℃ and 34℃. The application of moderate hyperthermia and hypothermia after LPS-induced cell activation attenuated the inflammatory response and reduced the likelihood of cell damage. These findings suggest that moderate temperature changes modulate the inflammatory response and could be a useful therapy against sepsis.These findings are available to treat inflammation of ventilator induced lung injury.

检测细胞培养温度的适度变化是否影响各种细胞因子和相关炎症介质的产生。在高温（40℃）、常温（37℃）和低温（34℃）条件下，用脂多糖（LPS）刺激小鼠巨噬细胞系RAW 264.7。然后检测细胞中热休克蛋白70（HSP 70）、热休克因子蛋白（HSF）和核因子-kB（NF-κ B）二聚体（p50和p65）的水平，以及培养上清液中高迁移率族蛋白1（HMG B1）和细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-β（IL-β）和白细胞介素-6（IL-6）的水平。与37℃相比，40℃和34℃下LPS刺激后HMGB 1、IL-1 β、IL-6和TNF-α以及NF-κ B二聚体（p50和p65）的水平均降低。在40℃和34℃时，HSP 70和HSF水平升高。在LPS诱导的细胞活化后应用中度高温和低温减弱了炎症反应并降低了细胞损伤的可能性。这些结果表明，适度的温度变化调节炎症反应，可能是一个有用的治疗脓毒症，这些研究结果可用于治疗呼吸机诱导的肺损伤的炎症。