Clinical application and functional analysis of the cofactor of androgen receptor in prostate cancer

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1) Establishment of androgen independent prostate cancer cell lineWe obtained the cell line that acquired the proliferation potency without androgen by culturing under charcoal stripped FBS for a long term. The anti-androgen medicine cannot show the growth inhibition effect in this cell line- We will analyze the cell line more closely in the future.2) Analysis of DNA methylation of the RIZ1 gene in prostate cancerRiz1 is a histone methyltransferase and a retinoblastoma protein-interacting zinc finger gene. DNA methylation of the RIZ1 gene was detected in 42.6% of prostate cancer tissues. DNA methylation of RIZ1 tended to be present more frequently in PCa specimens with a high Gleason score than in those with a low Gleason score. PC3 cell line showed loss of RIZ1 mRNA due to DNA methylation, and this loss was rectified by treatment with a demethylating agent, 5-Aza-2-deoxycytidine. These results suggest that transcriptional inactivation of RIZ1 by aberrant DNA methylation may contribute to prostate carcinogenesis.3) Estrogen related receptor (ERR) and Guanine nucleotide binding protein-like3 (GNL3L)We found out that the interaction between ERR and GNL3L. ERR and GNL3L express in prostate and prostate cancer tissue. GNL3L inhibits the transcriptional activity of ERR3 in prostate cancer cells. We will research a physiological function of ERRs and GNL3L in the prostate cancer cell in the future.