权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Expression and immunoreactivity of high-risk human papillomavirus E4 prtein

高危人乳头瘤病毒E4蛋白的表达和免疫反应性

基本信息

批准号：
18591827
负责人：
SASAGAWA Toshiyuki
金额：
$ 2.51万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591827/
关键词：
Human papillomavirus E4 protein Immunostaining Therepeutic HPV vaccine Diagnosis of HPV infection 免疫組織染色 HPV-DNAタイピング免疫応答

项目摘要

Certain human papillomavirus types named high-risk types are thought to cause cervical cancer and cervical intraepithelial neoplasia (CIN). It has been recently known that E4 proteins of these types are abundantly expressed in CIN epithelium. However, such expression has not been investigated extensively in clinical samples. This study is to examine the expression pattern using E4 antibodies for specific HPV types.We obtained seven different polyclonal antibodies against E4 proteins of HPV types 11, 16, 18, 31, 33, 56, which were produced from immunized rabbits and purified. lb start this experiment, we tried to do HPV genotyping in cytological samples using three different HPV genotyping methods in women who were abnormal in cytology. These methods are now commercially available in Japan. We found that different assays have shown discordant results in some of the samples examined. Discordant results were more frequently observed in the cases infected with different HPV types. This type of multiple HPV type infection were more common in women having abnormal cytology as we have previously expected. Now we are going to analyze more detail in these eases showing discordant results. This is a crucial step for further experiment for E4 study.E4 expression has been confirmed on epithelium from middle to superficial layers in most of CIN1 and CIN2, 3 lesions in which HPV types were determined. Abundant expression was seen in some cases. However, some immune cells were also stained by these antibodies. We have to figure out the positive reaction to immune cell is merely due to cross-reaction or due to presence of E4 in these immune cells. Furthermore, we have to test type-specificity of these antibodies in immune-staining. E4 protein immunostaining method may be useful for diagnostic purpose of high-risk HPV infection, and furthermore E4 might be a good target for therapeutic HPV vaccine.

某些被称为高危型的人乳头瘤病毒类型被认为会导致宫颈癌和宫颈上皮内瘤变（CIN）。最近已经知道，这些类型的E4蛋白在CIN上皮中大量表达。然而，这种表达尚未在临床样品中广泛研究。本研究采用免疫家兔的方法，制备了7株抗HPV11、16、18、31、33、56型E4蛋白的多克隆抗体，并进行了纯化。在本实验开始时，我们尝试在细胞学异常的妇女中使用三种不同的HPV基因分型方法对细胞学样品进行HPV基因分型。这些方法现在在日本已经可以买到了。我们发现，不同的检测方法在一些检测样本中显示出不一致的结果。不同型别HPV感染者的检测结果不一致。正如我们之前所预期的那样，这种类型的多重HPV感染在细胞学异常的女性中更常见。现在，我们将分析更多的细节，在这些情况下，显示不一致的结果。E4在HPV分型阳性的CIN 1、CIN 2、CIN 3病变中的中、浅层上皮均有表达。在某些病例中观察到大量表达。然而，一些免疫细胞也被这些抗体染色。我们必须弄清楚对免疫细胞的阳性反应仅仅是由于交叉反应或由于这些免疫细胞中存在E4。此外，我们必须在免疫染色中测试这些抗体的类型特异性。E4蛋白免疫组化方法可用于高危型HPV感染的诊断，并可作为治疗性HPV疫苗的良好靶点。