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The development of serological test detecting antibodies against oncogenic human papillomaviruses by ELISA

ELISA检测致癌人乳头瘤病毒抗体的血清学检测方法的开发

基本信息

批准号：
08671877
负责人：
SASAGAWA Toshiyuki
金额：
$ 1.54万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Immunoglobulins A and G (IgA and IgG) responses against HPV 16 virus-like particles (VLP) were tested by ELISA in 100 women with cervical lesions, 26 atypical cells of undetermined significance (ASCUS) and 14 cytologically normal women with HPV DNA.As controls, 130 age-matched cytologically normal women with no HPV DNA were selected from the population in which the cases generated. The existence of HPV DNA in cervical samples were tested by PCR-based method. Normal women positive with HPV 16 DNA were followed up at 4-7 months intervals for 16-24 months. IgA and IgG antibodies against HPV 16-VLP were frequently detected in these women repeatedly positive for HPV16 DNA,suggesting that the persistent HPV infection is crucial for effective antibody responses against the viruses. IgA response appears earlier and persist longer that IgG response. Women with HPV DNA of types 16,31/33/35,58 and unknown types showed significantly higher seropostivity for both IgA and IgG antibodies than the controls (P<0.05 for both). No significant seropositivity for IgA nor IgG was detected in HPV 18/45-DNA positive group. HPV 31/33/35,58 appear to be close types to HPV 16, whereas HPV 18/45 to be distinct to HPV 16 in antigenicity. IgA and IgG responses against HPV 16-VLP were more frequently observed in women with normal cervices with HPV DNA,ASCUS,HSIL and cervical cancer than in the controls. High serological responses were dependent on HPV 16-infection in HSIL and cervical cancer cases, although the association between serological responses and HPV types were not apparent in the more beign changes such as ASCUS and LSIL.Antibody positive reflects persistent viral infection that may increase the risk for malignant progression of the cervix. Thus this serological assay using HPV 16-VLP may be useful as a new diagnostic tool supplementing cervical cytological tests.

用ELISA法检测了100例宫颈病变、26例意义不明的非典型细胞（ASCUS）和14例HPV DNA阳性的正常宫颈组织对HPV 16病毒样颗粒（VLP）的免疫球蛋白A（伊加）和G（IgG）反应，并与130例无HPV DNA阳性的正常宫颈组织作对照。用PCR方法检测宫颈标本中HPV DNA的存在。对HPV 16 DNA阳性的正常妇女每隔4-7个月随访16-24个月。在这些HPV 16 DNA反复阳性的妇女中经常检测到针对HPV 16-VLP的伊加和IgG抗体，这表明持续的HPV感染对于针对病毒的有效抗体应答至关重要。伊加反应比IgG反应出现更早，持续时间更长。HPV 16、31/33/35、58型及未知型别妇女伊加、IgG抗体阳性率均显著高于对照组（P均<0.05）。HPV 18/45-DNA阳性组中伊加和IgG均无明显阳性。HPV 31/33/35，58型与HPV 16型相似，HPV 18/45型与HPV 16型抗原性不同。HPV 16-VLP的伊加和IgG反应在正常宫颈、ASCUS、HSIL和宫颈癌患者中较对照组更常见。在HSIL和宫颈癌病例中，高血清学应答依赖于HPV 16感染，尽管在更良性的变化如ASCUS和LSIL. Ab阳性反映持续的病毒感染可能增加宫颈恶性进展的风险中，血清学应答和HPV类型之间的关联不明显。因此，这种血清学检测HPV 16-VLP可能是有用的，作为一种新的诊断工具，补充宫颈细胞学检查。