HPV塩基配列決定による新規ハイリスクバリアントと高齢者子宮頸癌関連型の同定

目的本研究旨在评估人乳头瘤病毒（HPV）类型和变异对宫颈癌发生的风险。在本研究中，我们测定了宫颈癌、非典型增生和正常对照组宫颈细胞的HPV序列，并评估了检测到HPV的风险。材料与方法测定宫颈癌组、非典型增生组和细胞学正常组（对照组）细胞的shpv序列。用一致的引物扩增细胞的HPV L1区序列，然后用测序引物直接测序。每个HPV类型和变异的风险评估是由对照组和癌组之间的优势比确定的。结果共鉴定出35种HPV型别，从6型到91型不等，其中未分类的有4种。其中33型和58型是老年人群的高危型。本研究共鉴定出85种HPV基因型。HPV型16、18、31、33、35、52、58是世界范围内流行的类型，有几种变体。其中，5个16型变异中有3个在浸润性癌中检出，18型的4个变异中有1个在浸润性癌中检出，31型的2个变异中有1个在浸润性癌中检出，33型的2个变异中有1个在浸润性癌中检出，35型的3个变异中有1个在浸润性癌中检出，52型的5个变异中有3个在浸润性癌中检出，58型的2个变异中有3个在浸润性癌中检出。虽然被归为高危型，但在浸润性癌中未检出45、51、53、56、59、66、68、73型至少一种变异。相反，在低危型HPV中，在浸润性癌中至少检测到54、90、91型的一种变异。

PurposeThis research is designed for risk evaluation of human papillomavirus (HPV) types and variants for cervical carcinogenesis. In this study, HPV sequences of cervical cells of cervical cancer, dysplasia, and normal control groups were determined, and evaluated risks of detected HPVs.Material and MethodsHPV sequences of cells of cervical carcinoma group, dysplasia group, and cytologically normal group (conrtol goup) were determined. The L1 region of HPV sequences of cells were amplified with consensus primer set, and subsequently, direct sequencing was performed using sequencing primers. Risk evaluation of each HPV types and variants was determined by odds ratios of the conrol group between the carcinoma group.ResultsThe number of dentified HPV types was thirty-five, ranging from type 6 to type 91, including unclassified four types. Among them, type 33 and type 58 were the high-risk types in the elderly population.Total of 85 genotypes of HPV were identified in this study. HPV types 16, 18, 31, 33, 35, 52, 58, world-wide popular types, have several variants. Among them, three out of five variants of type 16, all four variants of type 18, one out of two variants of type 31, one out of two variants of type 33, one out of three variants of type 35, three out of five variants of type 52, all two variants of type 58 were detected in the invasive carcinoma. Although classified into the high-risk HPV, at least one variants of types 45, 51, 53, 56, 59, 66, 68, 73 were not detected in the invasive carcinoma. Reversely, classified into the low-riks HPV, at least one variants of types 54, 90, 91 were detected in the invasive carcinoma.