HPV塩基配列決定による新規ハイリスクバリアントと高齢者子宮頸癌関連型の同定

本研究旨在评估人乳头瘤病毒(HPV)类型及其变异在宫颈癌发生中的风险。本研究对宫颈癌组、不典型增生组和正常对照组宫颈细胞进行了HPV基因序列检测，并对检测到HPV的危险性进行了评估。用通用引物扩增细胞HPV序列的L1区，然后用测序引物进行直接测序。结果确诊的HPV型35例，从6型到91型不等，包括未分类的4种类型。其中33型和58型是老年人群的高危型，共鉴定出85种HPV基因型别。HPV16、18、31、33、35、52、58是世界范围内流行的类型，有几个变种。其中，在浸润性癌中检出16型5个变异体中的3个，18型的4个变异体中的3个，31型中的2个变异体中的1个，33型中的2个变异体中的1个，35型中的3个变异体中的1个，52型中的5个变异体中的3个，58型中的所有2个变异体中的3个。虽然被归类为高危HPV，但至少有一种45、51、53、56、59、66、68、73型变异在浸润性癌中未被检测到。相反，被归类为低RIKS的HPV，在浸润性癌中至少检测到54、90、91型的一个变异。

PurposeThis research is designed for risk evaluation of human papillomavirus (HPV) types and variants for cervical carcinogenesis. In this study, HPV sequences of cervical cells of cervical cancer, dysplasia, and normal control groups were determined, and evaluated risks of detected HPVs.Material and MethodsHPV sequences of cells of cervical carcinoma group, dysplasia group, and cytologically normal group (conrtol goup) were determined. The L1 region of HPV sequences of cells were amplified with consensus primer set, and subsequently, direct sequencing was performed using sequencing primers. Risk evaluation of each HPV types and variants was determined by odds ratios of the conrol group between the carcinoma group.ResultsThe number of dentified HPV types was thirty-five, ranging from type 6 to type 91, including unclassified four types. Among them, type 33 and type 58 were the high-risk types in the elderly population.Total of 85 genotypes of HPV were identified in this study. HPV types 16, 18, 31, 33, 35, 52, 58, world-wide popular types, have several variants. Among them, three out of five variants of type 16, all four variants of type 18, one out of two variants of type 31, one out of two variants of type 33, one out of three variants of type 35, three out of five variants of type 52, all two variants of type 58 were detected in the invasive carcinoma. Although classified into the high-risk HPV, at least one variants of types 45, 51, 53, 56, 59, 66, 68, 73 were not detected in the invasive carcinoma. Reversely, classified into the low-riks HPV, at least one variants of types 54, 90, 91 were detected in the invasive carcinoma.