The analysis of the stria vascularis and the lateral wall of cochlea in the audiometric hearing loss animal model by the Arg-Vasopressin

1.动物模型研究：我们先前的研究证实，给予精氨酸加压素(Arg-Vasopressin，AVP)的动物模型听力受损。对AVP动物模型的耳蜗侧壁，尤其是血管纹进行了形态学观察。Wistar大鼠腹腔注射AVP(0.02单位/g)。观察注射AVP后1小时内血管纹的形态变化。结果发现，在血管纹的中间细胞内有细胞内水肿。推测AVP引起的听力损伤与中间细胞的形态改变有关。认为细胞内水肿导致血管纹血液循环受损，氧分压降低，离子代谢受损。因此，人们认为听力受损了。临床研究。我们检查了是否足够的…对于梅尼埃病(MD)患者来说，更多的NT水摄入在长期控制眩晕和听力活动方面是有效的，因为传统疗法被证明对这些患者无效。具有历史控制的时间序列研究。第1组58例MD患者每日饮水35ml/kg，连续2年。2 9例在同一家医院接受常规饮食和利尿剂治疗2年以上的MD患者纳入2组的历史对照。在研究的最后6个月中，组1的患者眩晕显著缓解，最差纯音平均频率(0.125.2 5、0.2 5和0.5 kHz)(低PTA)的听力显著改善。相反，第二组患者在四个频率(0.5，1，2和4 kHz)的PTA和低PTA方面都变得更差，尽管他们的眩晕有所改善。1组听力改善18例，不变37例，差3例；2组听力改善11例，差16例。有意识地调节水的摄入量可能是治疗MD患者的最简单和最具成本效益的治疗方法。需要更大的研究来证实这些结果在更大的患者队列中。较少

1. Animal model study.In our previous study, it was confirmed that the hearing of the animal modes administered Arg-Vasopressin (AVP) was impaired. The lateral wall of cochlea, especially the stria vascularis in the animal model administered AVP was investigated morphologically. Wistar rats were intra-peritoneally injected AVP (0.02 units/g). The morphology in the stria vascularis in one hour after the administering AVP was investigated in electron microscopy. As the results, an intra-cellar edema was found in the intermediate cell of the stria vascularis. It is assumed that the hearing impairment induced by AVP administering is related to the morphological change in the intermediate cells. It is considered that the intra-cellular edema induce that a blood circulation in the stria vascularis is impaired, the oxygen partial pressure is decreased and the ion-metabolism is impaired. As the results, it was assumed that the hearing was impaired.2. Clinical study.We examined whether sufficie … More nt water intake is effective in the long-term control of vertigo and hearing activity in patients with Meniere's disease (MD) for whom conventional therapy has proven unsuccessful. Time-series study with historical control. Fifty-eight patients with MD in Group 1 drank 35 ml/kg/day of water for 2 years. Twenty-nine patients with MD treated with the conventional dietary and diuretic therapy for more than 2 years during 1992-99 at the same Hospital were enrolled in a historical control of Group 2. Patients in Group 1 dramatically relieved vertigo and significantly improved in the hearing of the worst pure-tone average of three frequencies (0.125, 0.25, and 0.5 kHz) (low-PTA) during the last 6 months of the study period. In contrast, patients in Group 2 became worse in both the four (0.5, 1, 2, and 4 kHz) frequency-PTA and the low-PTA, although their vertigo did improve. The number of patients whose hearing were improved, unchanged and worse were 18, 37 and 3 in Group 1 and 2, 11 and 16 in Group 2, respectively. Deliberate modulation of the intake of water may be the simplest and most cost-effective medical treatment for patients with MD.Larger studies will be needed to confirm these results in a larger patient cohort. Less