The analysis of the stria vascularis and the lateral wall of cochlea in the audiometric hearing loss animal model by the Arg-Vasopressin

1. 动物模型研究。在我们之前的研究中，证实了给药arg - vas加压素（AVP）的动物模式听力受损。对AVP动物模型耳蜗外侧壁特别是血管纹进行形态学观察。Wistar大鼠腹腔注射AVP（0.02单位/g）。电镜观察AVP给药1小时后血管纹的形态变化。结果发现血管纹中间细胞胞内水肿。推测AVP引起的听力损伤与中间细胞的形态改变有关。认为细胞内水肿导致血管纹血液循环受损，氧分压降低，离子代谢受损。根据结果，假定听力受损。临床研究。我们研究了是否足够的…更多的水摄入是有效的长期控制眩晕和听力活动的患者的梅尼埃病（MD），传统治疗已被证明是无效的。具有历史控制的时间序列研究。第1组58例MD患者连续2年饮水35 ml/kg/d。1992- 1999年在同一家医院接受常规饮食和利尿剂治疗2年以上的29例MD患者被纳入历史对照组2。在最后6个月的研究期间，第一组患者显著缓解了眩晕，并显著改善了三个频率（0.125,0.25和0.5 kHz）（低pta）的最差纯音平均听力。相比之下，第二组患者在四种频率（0.5、1、2和4 kHz） - pta和低pta中都变得更糟，尽管他们的眩晕有所改善。听力改善、不变和恶化的患者分别为1组18例、37例和3例，2组11例和16例。刻意调节饮水量可能是md患者最简单、最具成本效益的治疗方法。需要在更大的患者群体中进行更大规模的研究来证实这些结果。少

1. Animal model study.In our previous study, it was confirmed that the hearing of the animal modes administered Arg-Vasopressin (AVP) was impaired. The lateral wall of cochlea, especially the stria vascularis in the animal model administered AVP was investigated morphologically. Wistar rats were intra-peritoneally injected AVP (0.02 units/g). The morphology in the stria vascularis in one hour after the administering AVP was investigated in electron microscopy. As the results, an intra-cellar edema was found in the intermediate cell of the stria vascularis. It is assumed that the hearing impairment induced by AVP administering is related to the morphological change in the intermediate cells. It is considered that the intra-cellular edema induce that a blood circulation in the stria vascularis is impaired, the oxygen partial pressure is decreased and the ion-metabolism is impaired. As the results, it was assumed that the hearing was impaired.2. Clinical study.We examined whether sufficie … More nt water intake is effective in the long-term control of vertigo and hearing activity in patients with Meniere's disease (MD) for whom conventional therapy has proven unsuccessful. Time-series study with historical control. Fifty-eight patients with MD in Group 1 drank 35 ml/kg/day of water for 2 years. Twenty-nine patients with MD treated with the conventional dietary and diuretic therapy for more than 2 years during 1992-99 at the same Hospital were enrolled in a historical control of Group 2. Patients in Group 1 dramatically relieved vertigo and significantly improved in the hearing of the worst pure-tone average of three frequencies (0.125, 0.25, and 0.5 kHz) (low-PTA) during the last 6 months of the study period. In contrast, patients in Group 2 became worse in both the four (0.5, 1, 2, and 4 kHz) frequency-PTA and the low-PTA, although their vertigo did improve. The number of patients whose hearing were improved, unchanged and worse were 18, 37 and 3 in Group 1 and 2, 11 and 16 in Group 2, respectively. Deliberate modulation of the intake of water may be the simplest and most cost-effective medical treatment for patients with MD.Larger studies will be needed to confirm these results in a larger patient cohort. Less