Expression analysis of molecules specific for development of autoimmune uveitis

自身免疫性葡萄膜炎发生特异性分子的表达分析

• 批准号: 18591935
• 负责人: TAKEUCHI Maseru
• 金额: $ 2.43万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591935/
• 关键词: Behcet's disease; uveitis; autoimmune disease; microarrav; chemokine; experimental autoimmune uveitis; EAU

In this study, we examined molecules which are increasing their gene expression in peripheral T cells of mice developing experimental autoimmune uveoretinitis (EAU) by immunization with interphotoreceptor retinoid-binding protein (IRBP).As the results, mRNA expression which increased more than 5 fold on both 7 and 14 days after immunization were cytokines associated with Th17 cells except for those of Thl cells, implicating that Th17 cells were also involved in the occurrence of EAU.Subsequently, we collected peripheral T cells from behcet's patient with uveitis, stimulated them with S-Ag and IRBP, and measured production of IFN-g, IL-17, IL-6, TNFa, and IL-10. Unexpectedly, positive rates of T cells reactive for S-Ag and IRBP were statistically not different between Behcet's patients and healthy controls. However, the amounts of several produced cytokines were more in Behcet's patients than in healthy controls, in which Behcet's patients produced more IFN-g, IL-17, and IL-6 by stimulation with IRBP, and more IFN-g and TNFa by stimulation with S-Ag than those of healthy controls.We continue this series of experiments, also using peripheral T cells obtained from Behcet's patients who have not had uveitis, sarcoidosis patients, and VKH patients, et. al.

In this study, we examined molecules which are increasing their gene expression in peripheral T cells of mice developing experimental autoimmune uveoretinitis (EAU) by immunization with interphotoreceptor retinoid-binding protein (IRBP).As the results, mRNA expression which increased more than 5 fold on both 7 and 14 days after immunization were cytokines associated with Th17 cells except for those of Thl cells, implicating that Th17细胞还参与了EAU的发生。随后，我们从Behcet的葡萄膜炎患者中收集了外围T细胞，用S-AG和IRBP刺激了它们，并测量了IFN-G，IL-17，IL-17，IL-6，IL-6，TNFA和IL-10的产生。出乎意料的是，在Behcet的患者和健康对照组之间，S-AG和IRBP反应性的T细胞的阳性速率在统计学上没有差异。然而，在Behcet的患者中，几种产生的细胞因子的量比在健康对照组中更多的是，在这种对照中，Behcet的患者通过用IRBP刺激IRBP刺激了IFN-G，IL-17和IL-6的量，而通过S-AG进行S-AG刺激的IFN-G和TNFA多于健康对照。结节病患者和VKH患者等。 al。

项目成果

Proteomic Surveillance of Retinal Autoantigens in Endogenous Uveoretinitis:Implication of EsteraseD and Brain Type Creatine Kinase as Novel Autoantigens

内源性葡萄膜视网膜炎中视网膜自身抗原的蛋白质组学监测：酯酶D和脑型肌酸激酶作为新型自身抗原的意义

• 发表时间: 2007
• 作者: Y.Okunuki;Y.Usui;T.Hattori;T.Kezuka;K.Nishioka;T.Kato;M.Takeuchi;M.Usui
• 通讯作者: M.Usui

実験的自己免疫性ぶどう膜網膜炎発症マウスの網膜、末梢血T細胞におけるケモカイン遺伝子の発現解析

实验性自身免疫性葡萄膜视网膜炎小鼠视网膜及外周血T细胞趋化因子基因表达分析

• 发表时间: 2006
• 作者: 竹内 大;臼井 嘉彦;竹内 礼;奥貫 陽子;服部 貴明;大井 桂子;慶野 博;毛塚 剛司;臼井 正彦
• 通讯作者: 臼井 正彦

タクロリムス眼内注入による実験的ぶどう膜炎における神経保護関連遺伝子の発現上昇

眼内注射他克莫司诱导的实验性葡萄膜炎神经保护基因表达增加

• 发表时间: 2007
• 作者: 大井 桂子;慶野 博;山川 直之;竹内 大;臼井 正彦;後藤 浩
• 通讯作者: 後藤 浩

Monokine Induced by Interferon γ(Mig)(CXCL9)in the Vitreous of Patients With Proliferative Diabetic Retinopathy

增殖性糖尿病视网膜病变患者玻璃体内干扰素γ(Mig)(CXCL9)诱导的单核因子

• 发表时间: 2007
• 作者: T.Tsuboi;Y.Usui;Y.Okunuki;M.Takeuchi;H.Minoda;T.Hattori;T.Iwasaki;H.Goto;M.Usui;Y.Wakabayashi
• 通讯作者: Y.Wakabayashi

内因性ぶどう膜炎による網膜組織障害で二次的に誘導される抗網膜抗体標的抗原の網羅的同定

内源性葡萄膜炎继发视网膜组织损伤抗视网膜抗体靶抗原的综合鉴定

• 发表时间: 2007
• 作者: 奥貫 陽子;臼井 嘉彦;服部 貴明;毛塚 剛司;西岡久 寿樹;加藤 智啓;竹内 大;臼井 正彦
• 通讯作者: 臼井 正彦