The role of endothelial VASP for neutrophils transendothelial migration during acute inflammation.

急性炎症期间内皮 VASP 对中性粒细胞跨内皮迁移的作用。

• 批准号: 18591976
• 负责人: SAITO Hajime
• 金额: $ 2.21万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591976/
• 关键词: endothelial cells; neutronhils; acute inflammation; transmigration

The transendothelial migration of neutrophils is a critical step in acute inflammation, which we previously showed to be regulated by endothelial myosin light chain kinase, Rho and Rho kinase are also key mediators of myosin light chain (MLC) phosphorylation, but their roles in neutrophil migration has not been investigated. In the present study, a transwell chamber migration assay system incorporating endothelial monolayer was used to examined the numbers of migrating neutrophils and endothelial vasodilator-stimulated phopphorylation, a protein responsible for controlling the geometry of actin-filament, at selected times during the neutrophil migration, in vitro. The results showed that pretreating endothelial cells with hydroxyethyl starch (HES) significantly diminished neutrophil migration, H89 (protein kinase A inhibitor) inhibited the effect of HES on neutrophil transendothelial migration, and VASP phosphorylation normally associated with the inhibition of neutriphil transendothelial migration. These data suggest that endothelial VASP regulate transendothelial neutrophil migration by modulating the cytoskeletal events that mediate such migration.

中性粒细胞的跨内皮迁移是急性炎症中的关键一步，我们先前证明，肌球蛋白轻链激酶，RHO和RHO激酶对此进行了调节，这也是肌球蛋白轻链（MLC）磷酸化的关键介体，但它们在中性粒细胞迁移中的作用尚未调查。在本研究中，使用含有内皮单层的Transwell腔室迁移测定系统检查中性粒细胞迁移期间，在中性粒细胞迁移期间，在中性粒细胞迁移期间，在选定的时间内，用于控制肌动蛋白 - 细胞的几何形状的迁移中性粒细胞和内皮血管舒张的数量。结果表明，用羟乙基淀粉（HES）预处理内皮细胞显着减少中性粒细胞迁移，H89（蛋白激酶A抑制剂）抑制了HES对中性粒细胞跨性粒细胞迁移的作用，并抑制了与中性抑制抑制抑制中性中性跨质体的抑制作用。这些数据表明，内皮VASP通过调节介导这种迁移的细胞骨架事件来调节跨内皮中性粒细胞的迁移。