The role of vascular endothelial HSP70 on acute lung injury

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The transendothelial migration of neutrophils is a critical step in acute inflammation. In the present study, to estimate the role of heat shock protein (HSP), a transwell chamber migration assay system incorporating endothelial monolayer was used to examine the numbers of migrating neutrophils. The expression of endothelial vasodilator-stimulated phosphoprotein (VASP), a protein responsible for controlling the geometry of actin-filament, was also estimated at selected times during the neutrophil migration, in vitro. The results showed that pretreating endothelial cells with hydroxyethyl starch (HES) significantly diminished neutrophil migration, quercetin (HSP70 inhibitor) inhibited the effect of HES on neutrophil transendothelial migration, and VASP phosphorylation normally associated with the inhibition of neutrophil transendothelial migration. These data suggest that endothelial VASP regulate transendothelial neutrophil migration by modulating the cytoskeletal events that associated with upstream HSP70 signal transduction mechanisms.