The role of commensal microflora in the inane immune response during infection

共生微生物群在感染过程中免疫反应中的作用

• 批准号: 18591988
• 负责人: SHIME Nobuaki
• 金额: $ 2.6万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591988/
• 关键词: Sepsis; Innate immunity; Commensal micorflora; Digestive tract; Antibiotics; Macrophage; 敗血症; 常在菌巣

We investigated the role of digestive commensal bacteria in the innate immune response in experimental mouse sepsis model. Experimental mice were fed by water including multiple antibiotics (streptomycin and cefotaxime) for three weeks. Significant decreases in the amount of commensal bacterial flora, as well as the changes in intestinal organic acid profiles were noted in the experimental group. The expressions of TLR-4 and TLR-2 receptors in the peritoneal macrophages assessed by flow-cytometry, have been significantly downregulated. The increase in plasma TNF-α level after lipopolysaccharide injection, mimicking clinical sepsis, has been attenuated. This attenuation was accompanied with the increases in the mortality. Those data suggests that commensal bacterial flora in the digestive tract might confer a significant role in modulating innate immune system. The immunomodulating effect of antimicrobials via the changes in commensal microflora should be considered in the sepsis treatment.

我们研究了消化共生菌在小鼠脓毒症模型先天免疫应答中的作用。实验小鼠用含多种抗生素（链霉素和头孢噻肟）的水喂养3周。实验组的共生菌群数量明显减少，肠道有机酸谱也发生了变化。流式细胞术检测发现，腹腔巨噬细胞中TLR-4和TLR-2受体的表达明显下调。脂多糖注射后血浆TNF-α水平升高，模拟临床脓毒症，已减弱。这种衰减伴随着死亡率的上升。这些数据表明，消化道中的共生菌群可能在调节先天免疫系统中起重要作用。在脓毒症的治疗中，应考虑抗菌剂通过改变共生菌群的免疫调节作用。

项目成果

Continuous negative extrathoracic pressure in children after congenital heart surgery

先天性心脏病术后儿童持续胸外压持续负压

• 发表时间: 2006
• 期刊: Crit Care Resusc 8(4)
• 作者: Shime N;et al.
• 通讯作者: et al.

Glycopeptide pharmacokinetics in current paediatric cardiac surgery practice

当前小儿心脏手术实践中的糖肽药代动力学

• 发表时间: 2007
• 期刊: Eur J Cardiothrorac Surg. 32(4)
• 作者: Shime;N;et. al.
• 通讯作者: et. al.

誤嚥関連肺障害に対するシベレスタットナトリウムの有効性〜過去の症例報告の集積から

西维来司他钠治疗误吸相关肺部疾病的疗效——基于过去的病例报告集

• 发表时间: 2006
• 期刊: 麻酔 55
• 作者: Dohi K;Satoh K;Shioda S;Aruga T;et al.;志馬伸朗ら
• 通讯作者: 志馬伸朗ら

重症感染症に対する抗菌療法〜de-escalationは行われているか?

严重感染的抗菌治疗 - 是否正在进行降级治疗？

• 发表时间: 2006
• 期刊: 日本外科感染症学会誌
• 作者: Dohi K;Satoh K;Shioda S;Aruga T;et al.;志馬伸朗ら;青木 克憲;志馬伸朗
• 通讯作者: 志馬伸朗

小児に対するNPPV〜急性期管理での使用を中心に項担当、急性期NPPV実践マニュアル

儿童 NPPV - 急性期 NPPV 实用手册，主要侧重于急性期管理的使用

• 发表时间: 2006
• 作者: Katsunori;Aoki;et. al.;志馬伸朗
• 通讯作者: 志馬伸朗