Alterations of bone microenvironment associated with oncogenesis of odontogenic epithelium

与牙源性上皮肿瘤发生相关的骨微环境的改变

• 批准号: 18592057
• 负责人: KUMAMOTO Hiroyuki
• 金额: $ 1.31万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592057/
• 关键词: odontogenic tumor; tumor progression; bone microenvironment

To clarify the possible roles of interactions with bone microenvironment in oncogenesis of odontogenic epithelium, expression of matrix-degrading proteinases, angiogenic factors, growth factors, and their associates was analyzed in ameloblastomas and malignancy ameloblastomas as well as in tooth germs.1. uPA, uPAR, PAI-1, and maspin: uPA was recognized predominantly in mesenchymal cells, uPAR was evident in epithelial cells, PAI-1 was found in both epithelial and mesenchymal cells, and maspin was expressed only in epithelial cells. Expression of uPA and uPAR tended to be higher in ameloblastic tumors than in tooth germs, while PAI-1 was slightly lower in ameloblastic tumors than in tooth germs. Maspin expression was significantly higher in ameloblastomas than in tooth germs.2. PD-ECGF/TP and angiopoietin1, 2: PD-ECGF/TP was expressed in mesenchymal cells, and showed higher reactivity in ameloblastomas than in tooth germs. Angiopoietin1 and 2 were expressed mainly in epithelial cells neighboring the basement membrane, and malignant ameloblastic tumors showed low angiopoietin1 expression and high angiopoietin2 expression as compared with tooth germs and ameloblastomas.3. IGF, PDGF, and their receptors: These molecules were expressed mainly in epithelial cells neighboring the basement membrane. Expression of IGF-II was higher in ameloblastomas than in tooth germs, and desmoplastic ameloblastomas showed high expression of IGF and the receptor. Malignant ameloblastic tumors showed high expression of PDGF and the receptor, and PDGF expression was higher in follicular ameloblastomas than in plexiform ameloblastomas.

为了阐明与骨微环境的相互作用在牙源性上皮肿瘤发生中的可能作用，分析了基质降解蛋白酶、血管生成因子、生长因子及其相关因子在成釉细胞瘤和恶性成釉细胞瘤以及牙胚中的表达. uPA、uPAR、派-1和maspin：uPA主要在间充质细胞中被识别，uPAR在上皮细胞中是明显的，派-1在上皮和间充质细胞中都被发现，而maspin仅在上皮细胞中表达。uPA和uPAR在成釉细胞肿瘤中的表达高于牙胚，而派-1在成釉细胞肿瘤中的表达略低于牙胚。Maspin在成釉细胞瘤中的表达显著高于牙胚. PD-ECGF/TP和angiopoietin 1，2：PD-ECGF/TP在间充质细胞中表达，在成釉细胞瘤中比在牙胚中表现出更高的反应性。Angiopoietin 1和Angiopoietin 2主要表达于基底膜附近的上皮细胞，与牙胚和成釉细胞瘤相比，恶性成釉细胞瘤中Angiopoietin 1表达较低，Angiopoietin 2表达较高. IGF、PDGF及其受体：这些分子主要表达于基底膜附近的上皮细胞。IGF-II在成釉细胞瘤中的表达高于牙胚，促结缔组织增生性成釉细胞瘤显示IGF和受体的高表达。恶性成釉细胞肿瘤显示PDGF及其受体的高表达，并且PDGF在滤泡性成釉细胞瘤中的表达高于丛状成釉细胞瘤。

项目成果

Immunohistochemical detection of PD-ECGF/TP and angiopoietins in ameloblastic tumors

成釉细胞肿瘤中 PD-ECGF/TP 和血管生成素的免疫组织化学检测

• 发表时间: 2006
• 期刊: Journal of Oral Pathology ＆ Medicine 35・10
• 作者: Kumamoto H;Ooya K
• 通讯作者: Ooya K

Immunohistochemical detection of MT1-MMP, RECK, and EMMPRIN in ameloblastic tumors

成釉细胞肿瘤中 MT1-MMP、RECK 和 EMMPRIN 的免疫组织化学检测

• 发表时间: 2006
• 期刊: Journal of Oral Pathology ＆ Medicine 35・6
• 作者: Kumamoto H;Ooya K
• 通讯作者: Ooya K