Alterations of bone microenvironment associated with oncogenesis of odontogenic epithelium

为了阐明与骨微环境的相互作用在牙源性上皮肿瘤发生中的可能作用，分析了基质降解蛋白、血管生成因子、生长因子及其相关因子在成釉细胞瘤和恶性成釉细胞瘤以及牙胚中的表达。UPA、uPAR、PAI-1和maspin：uPA主要见于间充质细胞，uPAR见于上皮细胞，PAI-1见于上皮细胞和间质细胞，maspin仅在上皮细胞表达。UPA和uPAR在成釉细胞肿瘤中的表达高于牙胚，而PAI-1在成釉细胞肿瘤中的表达略低于牙胚。结论：1.Maspin在成釉细胞瘤中的表达明显高于在牙胚中的表达。PD-ECGF/TP和Angiopoietin1，2：PD-ECGF/TP在间充质细胞中表达，成釉细胞瘤的反应性高于牙胚。血管生成素1和血管生成素2主要表达于基底膜附近的上皮细胞，恶性成釉细胞瘤与牙胚和成釉细胞瘤相比，血管生成素1低表达，血管生成素2高表达。IGF、PDGF及其受体：这些分子主要在基底膜附近的上皮细胞表达。IGF-II在成釉细胞瘤中的表达高于在牙胚中的表达，结缔组织增生性成釉细胞瘤中IGF及其受体呈高表达。恶性成釉细胞瘤中PDGF及其受体高表达，滤泡性成釉细胞瘤的表达高于丛状成釉细胞瘤。

To clarify the possible roles of interactions with bone microenvironment in oncogenesis of odontogenic epithelium, expression of matrix-degrading proteinases, angiogenic factors, growth factors, and their associates was analyzed in ameloblastomas and malignancy ameloblastomas as well as in tooth germs.1. uPA, uPAR, PAI-1, and maspin: uPA was recognized predominantly in mesenchymal cells, uPAR was evident in epithelial cells, PAI-1 was found in both epithelial and mesenchymal cells, and maspin was expressed only in epithelial cells. Expression of uPA and uPAR tended to be higher in ameloblastic tumors than in tooth germs, while PAI-1 was slightly lower in ameloblastic tumors than in tooth germs. Maspin expression was significantly higher in ameloblastomas than in tooth germs.2. PD-ECGF/TP and angiopoietin1, 2: PD-ECGF/TP was expressed in mesenchymal cells, and showed higher reactivity in ameloblastomas than in tooth germs. Angiopoietin1 and 2 were expressed mainly in epithelial cells neighboring the basement membrane, and malignant ameloblastic tumors showed low angiopoietin1 expression and high angiopoietin2 expression as compared with tooth germs and ameloblastomas.3. IGF, PDGF, and their receptors: These molecules were expressed mainly in epithelial cells neighboring the basement membrane. Expression of IGF-II was higher in ameloblastomas than in tooth germs, and desmoplastic ameloblastomas showed high expression of IGF and the receptor. Malignant ameloblastic tumors showed high expression of PDGF and the receptor, and PDGF expression was higher in follicular ameloblastomas than in plexiform ameloblastomas.