2G allile of MMP-1 gene polymorphism increases risk of oral cancer

为了建立一种有效的骨再生补偿牙槽嵴畸形或低牙槽嵴的方法，我们尝试将自体培养的骨膜细胞与骨移植物结合使用。全身性骨膜细胞培养，由专职技术人员和盐委员会组成的生物无尘室，于2006年进行了自体培养骨膜在牙槽骨增强中的临床试验。(1)系统骨膜细胞培养条件：生物洁净室建设及专职培养技术人员培训；建立道德和软委员会（2006年）。(2)临床前试验培养：在实际培养系统中对培养程序、血清和培养基等试剂进行评估。检测细胞的生长和形态、细菌或病毒或微质感染情况以及犬血清残留水平。试验培养未见异常。在开始培养的第6周，观察到生长停滞。在培养的第一个阶段，通过巢式pcr分析检测到微质体污染，但在培养结束时消失。用免疫荧光法和斑点法检测，牛血清残留物均在检测限内。没有肿瘤形态学，如结节生长或异型性，估计。在整个培养过程中，ALP活性保持不变，表明成骨细胞表型一直维持到培养结束。(3)在项目实施期间，共进行了7例自体骨膜细胞移植。牙槽静脉骨移植3例，窦底抬高4例，辅以自体骨膜细胞。7例中2例已行假体治疗，无异常愈合过程，也无移植物感染的报道。移植区域的CT图像显示骨形成发育良好，移植物吸收较少。少

Tb establish a effective procedure of bone regeneration compensating deformed or low alveolar ridge for dental implantology, we attempted the administration of autologous cultured periosteal cell with the bone graft. Conditoning of systemic periosteal cell culture, in tearms of a biological clean room with full-time tecnitian and committee for the salty, in 2006 was followed by clinical test of the autologous cultured periosteum in augmentation of alveolar bone.(1) Conditoning of systemic periosteal cell culture: Construction of the biological clean room and training of full-time tecnitian for the culture; establishment of ethical and softy committees (2006).(2) Pre-clinical test sultures: estimation of the culture procedure, serum and mediums, and other reagents were performed in the actual culture system. Growth and morphology of cells, bacterial or virus or micoplasm infections, and remnant level of the bobine serum were examined. No abnormal finding was observed in the test culture … More . Growth arrest was observed on sixth week after the onset of cram At the first periods of the culture, contamination of micoplasms were detected by nested-PCR analysis, but were disappeared at the end of the culture. Bovine serum remnant was under the detection limit by the immunofluorosent or dot-blot methods. No oncologic morphology, such as nodal growth or atypism, was estimated. ALP activity was maintained through the culture process, suggesting osteoblastic phenotype was maintained until the end of the culture.(3) seven cases of autologous periosteal cell graft were experienced within the period of the project. Three of alveolar venia bone grafts, four cases of sinus floor elevations were supplemented by autologous periosteal cells. Two of the seven cases have been performed with prosthetic treatment No abnormal healing process, as well as no infection of the grafts was reported. CT image of the grafted area suggested well developed bone formation with less resorption of the graft. Less