Study of a Rapid Test for Mycobacterium tuberculosis Complex Using an Immunochromatographic Assay

使用免疫层析法快速检测结核分枝杆菌复合体的研究

• 批准号: 23659507
• 负责人: NAGATA Masaki
• 金额: $ 2.33万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659507/
• 关键词: 感染症診断学; 結核; イムノクロマト; 迅速診断; 感染症

We investigated simple and rapid test for M. tuberculosis complex by using immunochromatographic assay (ICA) to detect MPB64. Several secreted mycobacterial proteins have been reported. Among these secreted proteins, MPB64, has been identified as a M. tuberculosis complex-specific secretory protein. This kit is the newly developed ICA for rapid discrimination between the M. tuberculosis complex and the mycobacteria other than M. tuberculosis (MOTT). Primary investigation, we study rapid identification of the M. tuberculosis complex in combination with the culture systems based on liquid media. In this method, we can easily detect the M. tuberculosis complex from MOTT. Secondary, we investigated direct testing method from clinical specimens. Specimens were placed into the test well of kits, after concentrating the sediments processed and decontaminated. Direct testing method could correctly detect between the M. tuberculosis complex and MOTT, specimens contain high volume of M. tuberculosiscomplex. With a few issue, this system is useful for the rapid isolation and detection of M. tuberculosis complex.

我们通过使用免疫层析法 (ICA) 检测 MPB64，研究了简单快速的结核分枝杆菌复合体检测方法。已报道了几种分泌的分枝杆菌蛋白。在这些分泌蛋白中，MPB64 已被鉴定为结核分枝杆菌复合体特异性分泌蛋白。该试剂盒是新开发的ICA，用于快速区分结核分枝杆菌复合群和结核分枝杆菌以外的分枝杆菌（MOTT）。初步调查中，我们研究了结合基于液体培养基的培养系统对结核分枝杆菌复合体的快速鉴定。在这种方法中，我们可以轻松地从 MOTT 中检测结核分枝杆菌复合体。其次，我们研究了临床标本的直接检测方法。将沉淀物经过浓缩处理和净化后，将标本放入试剂盒的测试孔中。直接检测方法可以正确检测结核菌群和MOTT，标本中含有大量结核菌群。该系统存在一些问题，可用于结核分枝杆菌复合体的快速分离和检测。