Pulification, structure and characterization of anti-tumor compounds from marine organisms and analysis of their mechanisms

(1)海洋生物的纯化和从海洋生物中寻找抗癌化合物。海洋生物活性物质的纯化、结构。海洋生物抗肿瘤化合物的分离及其对口腔鳞状细胞癌细胞的细胞毒性评价。收集日本邻近海域的海洋生物并进行冷冻干燥。在乙酸乙酯-甲醇（4:1）中均质。高效液相色谱法将它们分布到己烷层、C2H5OCH3CO层和水层中。接着，用宾法测定了它们的活性，并对它们进行了纯化和结构测定。(2)对鳞状细胞癌细胞的抗肿瘤活性分析用无血清生物试验研究了从海洋生物中纯化的活性生物物质对人类癌细胞的抗肿瘤作用。海洋生物抗血管生成作用分析。肿瘤细胞附着、侵袭和转移的影响分析。对法尼基化的抑制分析。(3)分析海洋生物对接种人癌细胞的抗肿瘤作用，研究海洋生物对人癌细胞（口腔鳞状细胞癌和A431）的体内抗肿瘤活性。将OSCC细胞接种于Balb-c/nu/nu裸鼠。在此过程中，一些化合物与载体腹腔注射。对这些化合物的抗肿瘤作用进行了评价。

(1) Purification of marine organisms and search of anti-cancer compounds from marine organisms.Purification, structure of active biological substances from marine organisms.Isolation of anti-neoplastic compounds from marine organisms and evaluation of their cytotoxity against oral squamous cell carcinoma cells. Marine organisms from Japanese adjacent Sea were collected and freeze-dried. They were homogenized in EtOH-MeOH(4:1).They were distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer., proceedingly, were investigated their activity by bin assay and were purificated and decided their structure. Nobel agents were derived from active biological substances improving the structure of their branches(2) Analysis of antitumor activity on squamous cell carcinoma cellsActive biological substances pulified from marine organisms were examined on the antitumor effect of human cancer cells with serum-free bio assay.Analysis of anti-angiogenetic effect of marine organisms.Analysis of the effect of tumor cell attachment, invasion and metastasis.Analysis of inhibition against Has farnesylation.(3) Analysis of the anti-tumor effect of marine organisms on inoculated human cancer cell Anti-tumor activity against human cancer cells (oral squamous cell carcinoma and A431) were investigated in vivo.OSCC cells were inoculated on Balb-c/nu/nu nude mices. Proceedingly, some compounds were injected with vehicle intraperitoneally.Anti-tumor effect of these compounds were consequently evaluated their tumor volume.