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Pulification, structure and characterization of anti-tumor compounds from marine organisms and analysis of their mechanisms

海洋生物抗肿瘤化合物的纯化、结构、表征及其作用机制分析

基本信息

批准号：
18592182
负责人：
YOSHIOKA Yukio
金额：
$ 2.53万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592182/
关键词：
marine organisms active biological substances oral squamous cell carcinoma anti-tumor activity angiogenesis

项目摘要

(1) Purification of marine organisms and search of anti-cancer compounds from marine organisms.Purification, structure of active biological substances from marine organisms.Isolation of anti-neoplastic compounds from marine organisms and evaluation of their cytotoxity against oral squamous cell carcinoma cells. Marine organisms from Japanese adjacent Sea were collected and freeze-dried. They were homogenized in EtOH-MeOH(4:1).They were distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer., proceedingly, were investigated their activity by bin assay and were purificated and decided their structure. Nobel agents were derived from active biological substances improving the structure of their branches(2) Analysis of antitumor activity on squamous cell carcinoma cellsActive biological substances pulified from marine organisms were examined on the antitumor effect of human cancer cells with serum-free bio assay.Analysis of anti-angiogenetic effect of marine organisms.Analysis of the effect of tumor cell attachment, invasion and metastasis.Analysis of inhibition against Has farnesylation.(3) Analysis of the anti-tumor effect of marine organisms on inoculated human cancer cell Anti-tumor activity against human cancer cells (oral squamous cell carcinoma and A431) were investigated in vivo.OSCC cells were inoculated on Balb-c/nu/nu nude mices. Proceedingly, some compounds were injected with vehicle intraperitoneally.Anti-tumor effect of these compounds were consequently evaluated their tumor volume.

(1)海洋生物的提纯和抗癌化合物的寻找；海洋生物活性生物物质的纯化、结构；海洋生物抗肿瘤化合物的分离及其对口腔鳞状细胞癌细胞的细胞毒作用。采集了日本邻近海域的海洋生物并进行了冷冻干燥。它们在乙醇-甲醇(4：1)中均一，用高效液相色谱法将它们分散到正己烷层、C2H5OCH3CO层和水层，然后用Bin法考察它们的活性，并对它们进行纯化和结构测定。从海洋生物中提纯的活性生物物质对人癌细胞的抗肿瘤作用采用无血清生物测定法。海洋生物抗血管生成作用的分析。肿瘤细胞附着作用的分析(3)海洋生物对接种的人癌细胞的抑瘤作用分析。体内研究了海洋生物对人癌细胞(口腔鳞状细胞癌和A431)的抑制作用。在此基础上，将部分化合物经腹膜腔内注射，评价其抗肿瘤作用。