Genetic diagnosis and therapy targeting the Wnt signaling pathway of oral cancer

研究了纤溶酶原激活剂/纤溶酶系统在口腔鳞状细胞癌（SCC）中e -钙粘蛋白表达和功能中的作用。用纤溶酶原处理SCC细胞减少Ca2+依赖性细胞聚集。SCC细胞聚集时表达E-cadherin。SCC细胞在膜上表达E-cadherin，并在培养基中以80 kDa的速度释放少量可溶性E-cadherin。在SCC细胞中加入纤溶酶原导致细胞膜上e -钙粘蛋白的数量减少，e -钙粘蛋白外膜蛋白的脱落增强。纤溶蛋白直接切割SCC细胞的e -钙粘蛋白，增强SCC细胞的运动性。这些结果提示，纤溶酶原激活剂/纤溶酶系统可能直接介导e -钙粘蛋白在口腔鳞状细胞中的蛋白水解过程，并可能通过下调e -钙粘蛋白介导的细胞-细胞粘连促进口腔鳞状细胞的进展。

The participation of plasminogen activator/plasmin system in the expression and function of E-cadherin was examined in oral squamous cell carcinoma (SCC) cells. Treatment of SCC cells with plasminogen reduced the Ca2+ -dependent cell aggregation. SCC cells expressed E-cadherin at the cell aggregation. SCC cells expressed E-cadherin at the membrane, and released a small amount of soluble E-cadherin at 80 kDa in the culture medium. Addition of plasminogen to SCC cells led to a decrease in the amount of E-cadherin of the cell membrane and the enhancement of the shedding of E-cadherin Ectodomein. Plasmin, directly cleaved E-cadherin of SCC cells and enhanced the motility of SCC cells. these results suggested that plasminogen activator/plasmin system might directly mediate the proteolytic processing of E-cadherin in oral SCC cells and that might facilitate the progression of oral SCC by downreguration of E-cadherin-mediated cell-cell adhesion.