Genetic diagnosis and therapy targeting the Wnt signaling pathway of oral cancer

口腔癌Wnt信号通路基因诊断及治疗

• 批准号: 15592113
• 负责人: YOSHIOKA Yukio
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592113/
• 关键词: β-Catenin; oral squamous cell carcinoma; Wnt signaling pathway; plasmin; plasminogen; E-cadherin; α2-anti-plasmin; metastasis; invasion; gene therapy

The participation of plasminogen activator/plasmin system in the expression and function of E-cadherin was examined in oral squamous cell carcinoma (SCC) cells. Treatment of SCC cells with plasminogen reduced the Ca2+ -dependent cell aggregation. SCC cells expressed E-cadherin at the cell aggregation. SCC cells expressed E-cadherin at the membrane, and released a small amount of soluble E-cadherin at 80 kDa in the culture medium. Addition of plasminogen to SCC cells led to a decrease in the amount of E-cadherin of the cell membrane and the enhancement of the shedding of E-cadherin Ectodomein. Plasmin, directly cleaved E-cadherin of SCC cells and enhanced the motility of SCC cells. these results suggested that plasminogen activator/plasmin system might directly mediate the proteolytic processing of E-cadherin in oral SCC cells and that might facilitate the progression of oral SCC by downreguration of E-cadherin-mediated cell-cell adhesion.

研究了纤溶酶原激活剂/纤溶酶系统在口腔鳞状细胞癌（SCC）中e -钙粘蛋白表达和功能中的作用。用纤溶酶原处理SCC细胞减少Ca2+依赖性细胞聚集。SCC细胞聚集时表达E-cadherin。SCC细胞在膜上表达E-cadherin，并在培养基中以80 kDa的速度释放少量可溶性E-cadherin。在SCC细胞中加入纤溶酶原导致细胞膜上e -钙粘蛋白的数量减少，e -钙粘蛋白外膜蛋白的脱落增强。纤溶蛋白直接切割SCC细胞的e -钙粘蛋白，增强SCC细胞的运动性。这些结果提示，纤溶酶原激活剂/纤溶酶系统可能直接介导e -钙粘蛋白在口腔鳞状细胞中的蛋白水解过程，并可能通过下调e -钙粘蛋白介导的细胞-细胞粘连促进口腔鳞状细胞的进展。

项目成果

Role of stromal thorombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cells

基质thorombospondin-1在口腔鳞状细胞癌细胞运动和蛋白水解活性中的作用

• 发表时间: 2003
• 期刊: Int J Mol Med 12(4)
• 作者: Hayashido;Y.
• 通讯作者: Y.

Plasminogen activator/plasmin system suppresses cell-cell adhesion of oral squamous cells via proteolysis of E-cadherin

纤溶酶原激活剂/纤溶酶系统通过 E-钙粘蛋白的蛋白水解抑制口腔鳞状细胞的细胞粘附

• 期刊: Int J Oncol (In press)
• 作者: Hayashido;Y.
• 通讯作者: Y.

Role of stromal thorombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cells.

基质 thorombospondin-1 在口腔鳞状细胞癌细胞运动和蛋白水解活性中的作用。

• 发表时间: 2003
• 期刊: Int.J.Mol.Med. 12(4)
• 作者: Hayashido;Y.
• 通讯作者: Y.

Zooxanthellactone, a Novel γ-Lactone-type Oxylipine from Dinoflagellates of Symbiodinium sp. : Structure, Distribution, and Biological Activity

虫黄内酯，一种来自共生甲藻的新型 γ-内酯型 Oxylipine：结构、分布和生物活性。

• 发表时间: 2004
• 期刊: Biosci.Biotechnol.Biochem. 68(4)
• 作者: Ken-ichi;O.
• 通讯作者: O.

Yasutaka H., Yukio Y.et al.: "Participation of fibroblasts in MMP-2 binding and activation on the surface of oral squamous cell"International Journal of oncology. 22. 657-662 (2003)

Yasutaka H.、Yukio Y.等人：“成纤维细胞参与口腔鳞状细胞表面 MMP-2 的结合和激活”国际肿瘤学杂志。