Proteomic analysis of molecular-targeted therapy against KGFR of salivary gland carcinomas

唾液腺癌 KGFR 分子靶向治疗的蛋白质组学分析

• 批准号: 18592184
• 负责人: TORATANI Shigeaki
• 金额: $ 2.52万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592184/
• 关键词: KGFR; carcinoma of salivary gland; differentiation; proteomic analysis; FGF; KGF; Salivary Adenocarcinoma; Differentiation; Proteome

KGF, one of fibroblast growth factor receptor, is a receptor gene of KGF/FGF-7. We clarified salivary gland tumors accompany the abnormal expression or over-expression of FGF-2 in the process of malignant alteration, furthermore, KGFR gene expression disappeared and FGFR1-IIIc gene, receptor gene of FGF-2 without expression in salivary gland epithelium usually, increase as the enlarging malignancy.In this study, gene expressions were analyzed systematically and exhaustively for salivary gland carcinoma cells transfected wild-type KGFR, gene and control carcinoma cells. And we analyzed not only construction or change in function of oncogene and tumor suppressor gene products but also expression level of interfaced proteins and modified change after translation. FGF2-FGFR1-IIIc system was blocked through inhibition of FGFR1-IIIc expression using shRNA expression vector. The proliferation potency of cells transferred FGFR1-IIIc siRNA decreased. The gene and protein groups by related cell differentiation and apoptosis inducted with manipulation of FGF-FGFR signal were analyzes by DNA micro array and proteome system.In the results, about 900 gene expressions of salivary gland carcinoma transferred wild type KGFR gene were increased. Although about 400 gene expressions were decreased. Variation of gene expressions related apoptosis, carcinogenesis, cell cycle and molecular transducer were recognized. Compared gene expression of cells transfected KGFR gene with cells transfferd FGFR1 siRNA, above 50 genes were overlapped Next proteomic analysis of protein group interlocking FGFR was performed. Above 80 protein spots varied twice were detected. In contrast, above 100 protein spots were changed by transfection of FGFR1 siRNA.

KGF是成纤维细胞生长因子受体之一，是KGF/FGF-7的受体基因。在恶性改变的过程中，FGF-2的异常表达或过表达阐明了唾液腺肿瘤，此外，KGFR基因表达消失，FGFR1-IIIIC基因，FGF-2的受体基因，FGF-2的受体基因，在唾液上表达的唾液中，不表达不表达这种疾病，并详尽地表达了疾病。腺癌细胞转染野生型KGFR，基因和对照癌细胞。而且，我们不仅分析了癌基因和肿瘤抑制基因产物功能的构建或变化，还分析了交换蛋白的表达水平以及翻译后的改性变化。 FGF2-FGFR1-IIIC系统通过使用SHRNA表达载体抑制FGFR1-IIIIC表达来阻止。细胞转移FGFR1-IIIC siRNA的增殖效力降低。通过DNA微阵列和蛋白质组系统分析了通过相关细胞分化和通过FGF-FGFR信号操纵的相关细胞分化和凋亡的基因和蛋白质组。在结果中增加了唾液腺癌的900个基因表达，增加了野生型kgfr基因的基因表达。尽管大约有400个基因表达降低。认识到基因表达相关的凋亡，癌变，细胞周期和分子传感器的变异。比较了细胞与细胞转染的基因的基因表达，对蛋白质组相互锁定FGFR的下一个蛋白质组学分析重叠了高于50基因的基因。检测到高于80的蛋白质斑点两次。相反，通过转染FGFR1 siRNA改变了100多个蛋白质斑点。

项目成果

Immunohistochemical expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFRBP-1) as an angiogenic factor in head and neck tumorigenesis.

肝素结合蛋白 17/成纤维细胞生长因子结合蛋白-1 (HBp17/FGFRBP-1) 作为头颈部肿瘤发生中的血管生成因子的免疫组织化学表达。

• 发表时间: 2007
• 期刊: Oncology Report 17
• 作者: Begum S;Zhang Y;Shintani T;Toratani S;Sato JD;Okamoto T.:
• 通讯作者: Okamoto T.:

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• DOI: 10.1016/j.ijcard.2020.03.015
• 发表时间: 2020
• 期刊: International Journal of Cardiology
• 影响因子: 3.5
• 作者: Yamamoto Keiko;Nishimura Rintaro;Kato Fumiaki;Naito Akira;Suda Rika;Sekine Ayumi;Jujo Takayuki;Shigeta Ayako;Sakao Seiichiro;Tanabe Nobuhiro;Tatsumi Koichiro
• 通讯作者: Tatsumi Koichiro

Immunohistochemical expression of heparin-binding protein 17/fibroblast growth factor-binding protein (HBpl7/FGFBP-1)as an angiogenic factor in head and neck tumorigenesis

肝素结合蛋白17/成纤维细胞生长因子结合蛋白（HBpl7/FGFBP-1）作为头颈部肿瘤发生中血管生成因子的免疫组织化学表达

• 发表时间: 2007
• 期刊: Oncol Rep. 17(3)
• 作者: Begum;S.;Zhang;Y.;Shintani;T.;Toratani;S.;Sato;JD.;Tetsuji Okamoto
• 通讯作者: Tetsuji Okamoto

Immunohistochemical expression of heparin-binding protein17/fib roblast growth factor-binding protein-1(HBp17/FGFRBP-1) as an angiogenic factor in head and neck tumorigenesis.

肝素结合蛋白 17/纤维母细胞生长因子结合蛋白 1（HBp17/FGFRBP-1）作为头颈部肿瘤发生中的血管生成因子的免疫组织化学表达。

• 发表时间: 2007
• 期刊: Oncology Report 17
• 作者: Begum S;Zhang Y;Shintani T;Toratani S;Sato J D;Okamoto T.
• 通讯作者: Okamoto T.

Immunohistochemical expression of heparin-binding protein 17/fibroblast growth factor-binding protein (HBp17/FGFBP-1) as an angiogenic factor in head and neck tumorigenesis.

肝素结合蛋白 17/成纤维细胞生长因子结合蛋白 (HBp17/FGFBP-1) 作为头颈部肿瘤发生中的血管生成因子的免疫组织化学表达。

• 发表时间: 2007
• 期刊: Oncology Reports 17
• 作者: S.Begum;Y.Zhang;et al.
• 通讯作者: et al.