ESTABLISHMENT OF TUMOR SPECIFIC MOLECULER TARGETTING THERAPY AGAINST HYPDXIA INDUCIBLE FACTOR

缺氧诱导因子肿瘤特异性分子靶向治疗的建立

• 批准号: 18592201
• 负责人: HATORI Masashi
• 金额: $ 2.49万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592201/
• 关键词: HYPOXIA; OSCC; CELL CYCLE; MMP; INVASIVENESS; 線維芽細胞

1.Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via G1 phase cell cycle wrest1) Hypoxia did not affect cell viability of OSCC cells up to 8 hour incubation2) Hypoxia did not affect cell proliferation of NA and SAS, whereas the proliferation ofHSC-3, CA9-22 was down-regulated.3) Hypoxia sensitive cells (HS) display enhanced expression of HIF-1 α within 24 h incubation in hypoxia chamber.4) Hypoxia resistant cells (HR) display constitutive expression of HIF-1α.5) HS showed G1 arrest under the hypoxic condition.6) Enhanced cyto-toxicity of 5-FU was observed in HS.2, Effects of hypoxia on cell invasiveness of OSCC cells1) Hypoxia enhances invasiveness of OSCC cells in Matrigel invasion assay.2) Hypoxia induces expression of MMP-2, MMP-9 of OSCC cells.3) Hypoxia ski induces secretion of MMPs in culture supernatant.4) Activated MMP-2 in OSCC cells was up-regulated by hypoxia5) Fibroblast conditioned medium cultured in hypoxia chamber enhanced MMP-2 expression.

1.缺氧通过G1期细胞周期诱导口腔癌细胞对5-氟尿嘧啶的耐药1)缺氧对OSCC细胞培养至8小时不影响细胞活力2)缺氧不影响NA和SAS细胞增殖，而下调hsc -3、CA9-22的增殖。3)缺氧敏感细胞（HS）在缺氧室培养24 h后HIF-1 α表达增强。4)耐缺氧细胞（HR）显示HIF-1α的组成性表达。5) HS在缺氧条件下出现G1骤停。2、缺氧对OSCC细胞侵袭性的影响1)在Matrigel侵袭实验中，缺氧可增强OSCC细胞的侵袭性。2)缺氧诱导OSCC细胞MMP-2、MMP-9的表达。3)缺氧诱导培养上清分泌MMPs。4)缺氧可上调OSCC细胞活化的MMP-2； 5)缺氧室培养成纤维细胞条件培养基可增强MMP-2的表达。

项目成果

低酸素環壌における口腔扁平上皮癌のHIF-1αの発現及び浸潤能の解析

低氧环境下口腔鳞癌HIF-1α表达及侵袭能力分析

• 发表时间: 2006
• 作者: 栗原 祐史;羽鳥 仁志;他
• 通讯作者: 他

Enhanced susceptibility to apoptosis of oral squamous cell carcinoma cells subjected to combined treatment with anticancer drugs and phosphatidylinositol 3-kinase inhibitors

抗癌药物与磷脂酰肌醇3激酶抑制剂联合治疗口腔鳞状细胞癌细胞凋亡敏感性增强

• 发表时间: 2007
• 期刊: Int J Oncol 31
• 作者: Iwase;M;Yoshiba;S;Uchida;M;Takaoka;S;Kurihara;Y;Ito;D;Hatori;M;Shintani;S
• 通讯作者: S

Thalidomide suppresses melanoma growth by activatiing natural killer cells in mice

沙利度胺通过激活小鼠自然杀伤细胞抑制黑色素瘤生长

• 发表时间: 2006
• 期刊: Oncology Report 16
• 作者: Kawamata;A;Ito;D;Odani;T;Isobe;T;Iwase;M;Hatori;M;Nagumo;M
• 通讯作者: M

Thalidomide suppresses melanoma growth by activating natural killer cells in mice.

• DOI: 10.3892/or.16.6.1231
• 发表时间: 2006-12
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: Ai Kawamata;D. Ito;Takeshi Odani;T. Isobe;M. Iwase;M. Hatori;M. Nagumo

頬脂肪体有茎弁移植による口蓋腫瘍切除後の再建:症例報告と文献的考察

颊脂肪垫带蒂皮瓣移植腭部肿瘤切除术后重建病例报告及文献复习

• 期刊: Dental Medicine Research (印刷中)
• 作者: 伊東 大典;羽鳥 仁志;他
• 通讯作者: 他