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Analysis of the protein markers and genetic markers to establish an early diagnostic systems for the oral cancer

分析蛋白质标记和遗传标记，建立口腔癌早期诊断系统

基本信息

批准号：
18592205
负责人：
OHSHIMA Tomoko
金额：
$ 2.45万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

1. Proteomic analysis for identification of the biomarkers in oral squamous cell carcinoma (OSCC) tissues:The tumor tissues of OSCC were collected as operation frozen-specimens from eight patients in the hospital attached to Tsurumi University, and the tumor and normal epithelial tissue of the same individuals were gained by the Laser Captured Microdissection method. The extracted proteins were analyzed by SELDI TOF-MS systems as differential proteome analysis, and the significantly increased or decreased peak patterns were detected. This result suggested these peaks should be the candidates for the biomarkers of OSCC.2. A search of risk diagnosis markers (genetic markers) of OSCC:The cancer is genetic abnormality, and it is thought that a hereditary factor controls it greatly whether it is cancer constitution. Therefore, an individual cancer risk diagnosis is necessary to realize the personal cancer prevention. We have searched the risk-marker genes by a case-control study of genome wide screening of the SNPs. The DNA was collected and purified, and the SNPs were detected by a micro-beads array systems. When the Chi-squared test was carried out on the difference of the distribution rate of 5984 SNPs between the 117 cases and 153 controls, 9SNPs of chromosome number 2,6,7,8,10,11,12,16,19 were shown as p<0.0001, 15SNPs were p<0.01, and 327SNPs were p<0.05. Next, we carried out the haplotyping using the EM algorism, it was shown that the 1353 SNPs participated in the haplotype-block formation, and the result of Chi-squared test between cases and controls showed the p-values of 24 blocks were less than 0.00001, but those were scattered on chromosomes.From the results of 1 and 2, it was shown that there are plural candidates of protein and genetic markers, and it was thought the combination of them might make significant as diagnostic markers.

1.蛋白质组学分析用于鉴定口腔鳞状细胞癌（OSCC）组织中的生物标志物：收集来自鹤见大学附属医院的8名OSCC患者的肿瘤组织作为手术冷冻标本，并通过激光捕获显微切割方法获得相同个体的肿瘤和正常上皮组织。采用SELDI TOF-MS系统对提取的蛋白质进行差异蛋白质组分析，检测到明显增加或减少的峰型。这一结果表明这些峰应该是OSCC的生物标志物的候选者。OSCC的风险诊断标记（遗传标记）的研究：癌症是遗传异常，被认为是遗传因素在很大程度上控制它是否是癌症体质。因此，个体癌症风险诊断是实现个人癌症预防的必要条件。我们通过全基因组SNP筛查的病例对照研究寻找风险标记基因。收集并纯化DNA，采用微珠芯片系统检测SNPs。对5984个SNPs在117例病例组和153例对照组中的分布率差异进行卡方检验，染色体数目为2、6、7、8、10、11、12、16、19的9个SNPs p<0.0001，15个SNPs p<0.01，327个SNPs p<0.05。接下来，我们使用EM算法进行单倍型分型，结果表明，1353个SNPs参与了单倍型区块的形成，病例组与对照组之间的卡方检验结果显示，24个区块的p值小于0.00001，但这些区块分散在染色体上。结果表明，存在多种蛋白质和遗传标记的候选物，并且认为它们的组合可能作为诊断标记而具有重要意义。