Molecular Mechanism of invasion in Oral squamous cell carcinomas.-small GTPase regulates the invasion

上皮-间质相互作用是转移性肿瘤细胞侵袭周围组织的关键。本文提出了一种改良的体外纤维连接蛋白降解/侵袭实验，即肿瘤细胞与成纤维细胞共培养。利用这种方法，我们估计了三个细胞系来自人口腔鳞状细胞癌不同的侵袭模式的侵袭活性。根据Yamamoto Kohama的分类，OSC 20为3级，OSC 19为4C级，HOC 313为4D级。与成纤维细胞共培养时，HOC 313细胞的侵袭力强于OSC 20和OSC 19细胞。此外，敲低癌细胞或/和成纤维细胞的MT 1-MMP完全抑制体外侵袭力。

Epithelial-mesenchymal interaction is crucial in invade surrounding tissues by metastatic tumor cells. Here, we presented modified an in vitro fibronectin degradation/invasion assay that was co-cultured tumor cell with fibroblast. Using this assay, we estimated invasive activity as to three cell lines derived from human oral squamous cell carcinomas with different invasion mode. According to Yamamoto Kohama's classification, OSC20 was classified into grade 3, OSC19 into grade 4C and HOC313 into grade 4D. HOC313 showed strong invasiveness in co-cultured with fibroblast than OSC20 and OSC19 did. Also, knock down for MT1-MMP of cancer cell or/and fibroblast completed inhibit in vitro invasiveness.