Enhancement of apoptosis in oral cancer cells by intracellular reactive oxygen amplication

细胞内活性氧扩增增强口腔癌细胞凋亡

基本信息

  • 批准号:
    18592214
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

The effects of various free radicals derived from 6-formylpterin (6-FP), alpha-phenyl-tert-butyl nitrone (PBN) and 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH) combined with hyperthermia, on gene expression in similarly enhanced apoptosis of human lymphoma U937 cells were investigated using cDNA microarrays containing approximately 16,600 genes and computational gene expression analysis tools. When the cells were treated for 10 min at 44 degrees C (15% apoptosis level), 39 up-regulated and 3 down-regulated genes were identified. In the up-regulated genes, apoptosis-and unfolded protein response-associated genes were contained. The combined treatment with heat and either chemical enhanced apoptosis level (approximately 30%) and showed a chemical-specific gene expression pattern. Furthermore, the expression levels of selected genes were confirmed by a real-time quantitative PCR. The present results will provide a basis for further understanding the molecular mechanisms in enhancement of heat-induced apoptosis by different intracellular oxidative stress.
利用含有约16600个基因的cDNA芯片和计算基因表达分析工具,研究了6-甲酰基蝶呤(6-FP)、α -苯基叔丁基硝基酮(PBN)和2,2-偶氮双盐酸(2-氨基丙烷)二盐酸(AAPH)自由基联合高温对人淋巴瘤U937细胞凋亡基因表达的影响。当细胞在44℃(15%凋亡水平)下处理10 min时,鉴定出39个上调基因和3个下调基因。在上调的基因中,包含凋亡和未折叠的蛋白反应相关基因。热和任何一种化学药物联合处理可提高细胞凋亡水平(约30%),并显示出化学特异性基因表达模式。此外,所选基因的表达水平通过实时定量PCR确认。本研究结果将为进一步了解不同细胞内氧化应激促进热诱导细胞凋亡的分子机制提供基础。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Pulp ablation therapy by inductive heating-heat generation characteristics in pulp cavity
感应加热牙髓消融治疗——牙髓腔发热特性
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    3.8
  • 作者:
    Wada;S.;Tazawa;K.;Suzuki;N.;Furuta;I.;Nagano;I
  • 通讯作者:
    I
Pulp ablation therepy by inductive heating-heat generation character istics in pulp cavity-.
感应加热牙髓消融治疗-牙髓腔内发热特性-。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wada S.;et. al.
  • 通讯作者:
    et. al.
A hydrogen peroxide-generating agent, 6-formylpterin, enhances heat-induced apoptosis
  • DOI:
    10.1080/02656730400025404
  • 发表时间:
    2005-05
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    S. Wada;Zheng-Guo Cui;Takashi Kondo;Qing‐Li Zhao;R. Ogawa;M. Shoji;Toshiyuki Arai;Keisuke Makino;Isao Furuta
  • 通讯作者:
    S. Wada;Zheng-Guo Cui;Takashi Kondo;Qing‐Li Zhao;R. Ogawa;M. Shoji;Toshiyuki Arai;Keisuke Makino;Isao Furuta
Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia
不同自由基发生器和热疗联合处理人淋巴瘤U937细胞凋亡增强中的基因表达
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wada;S.;Tabuchi;Y.;Kondo;T.;Cui;Z-G.;Zhao;Q-L.;Takasaki;I.;Salunga;TL.;Ogawa;R.;Arai;T.;Makino;K.;Furuta;I
  • 通讯作者:
    I
Gene exoression ln enhanced apoptosis of human lyphoma U937 cells treated with the combination of different free radical generators and hyperthermia
不同自由基发生器和热疗联合处理的人淋巴瘤U937细胞凋亡中的基因外排增强
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wada S.;et. al.
  • 通讯作者:
    et. al.
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WADA Shigehito其他文献

WADA Shigehito的其他文献

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{{ truncateString('WADA Shigehito', 18)}}的其他基金

Oral cancer therapy by differential temperature control using nano-particle
使用纳米颗粒进行温差控制的口腔癌治疗
  • 批准号:
    20592355
  • 财政年份:
    2008
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Enhancement of radiation and hyperthermia effects for oral cancer by intracellular hydrogen peroxide generator
细胞内过氧化氢发生器增强口腔癌的放射和热疗效果
  • 批准号:
    16591987
  • 财政年份:
    2004
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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  • 批准号:
    19K12338
  • 财政年份:
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关注氧化应激的生活环境因素与低剂量辐射健康影响比较研究
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