Application ofnovel cell patterning technology for capillary engineering to connective tissue graft
毛细血管工程新型细胞图案化技术在结缔组织移植中的应用
基本信息
- 批准号:18592259
- 负责人:
- 金额:$ 2.62万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The purpose of our study was to gather information required for application of novel cell patterning technology for capillary engineering to establish and modify connective tissue graft procedure.1. Establishment of in vivo evaluation system of bioengineered capillary 1) We histologically confirmed the existence of red blood cells in the bioengineered capillaries when grafted in ear flap of nude moice. 2) We measured blood low using laser-Doppler flowmetry and confirmed that the functional bioengineered capillaries in mouse hind limb ischemia model.2. Isolation of endothelial ells from human peripheral blood and omentum majus We succeeded to isolate endothelial cells and endothelial progenitor cells from human omentum majus and peripheral blood, respectively. We also established cell culture condition for in vitro cell patterning.3. Isolation of multi-potent cells from periodontal ligament 1) Success in isolation of neurospere-forming cells from rat periodontal ligament. 2) Isolation of stem cells from human periodontal ligament. Real-time PCR analysis showed various stem cell markers. 3) Establish real-time PCR condition for markers of stern cell, cementum, and periodontal ligament cells.We are currently investigating the effects of the multi-potent cells derived from human periodontal on periodontal regeneration in nude rat.
我们研究的目的是收集用于毛细血管工程的新型细胞构图技术的应用所需的信息,以建立和修改结缔组织移植程序。生物工程毛细血管体内评价体系的建立1)组织学证实,将生物工程毛细血管移植到裸鼠耳皮瓣内,可见有红细胞存在。2)用激光多普勒血流仪测定小鼠的血流量,证实生物工程功能性毛细血管在小鼠后肢缺血模型中的作用。人外周血和人大网膜内皮细胞的分离我们成功地从人大脐和外周血中分离出内皮细胞和内皮祖细胞。建立了细胞体外培养条件,为细胞构型奠定了基础。牙周膜多能细胞的分离1)成功地从大鼠牙周膜分离出神经精子形成细胞。2)人牙周膜干细胞的分离。实时定量聚合酶链式反应分析显示了多种干细胞标志物。3)建立人牙周干细胞、牙骨质细胞和牙周膜细胞标志物的实时荧光定量聚合酶链式反应条件。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Extracellular signal-regulated kinase 1/2 is involved in ascorbic acid-induced osteoblastic differentiation in periodontal ligament cells
- DOI:10.1902/jop.2007.060223
- 发表时间:2007-02-01
- 期刊:
- 影响因子:4.3
- 作者:Mimori, Kaori;Komaki, Motohiro;Ishikawa, Isao
- 通讯作者:Ishikawa, Isao
A comparison of the tube forming potentials of early and late endothelial progenitor cells
- DOI:10.1016/j.yexcr.2007.11.016
- 发表时间:2008-02-01
- 期刊:
- 影响因子:3.7
- 作者:Mukai, Nana;Akahori, Taichi;Morita, Ikuo
- 通讯作者:Morita, Ikuo
Stem cell properties of human periodontal ligament cells
- DOI:10.1111/j.1600-0765.2006.00870.x
- 发表时间:2006-08-01
- 期刊:
- 影响因子:3.5
- 作者:Nagatomo, K;Komaki, M;Ishikawa, I
- 通讯作者:Ishikawa, I
Cyclooxygenase-2-dependent prostaglandin E (2) upregulates interleukin (IL) -lalpha-induced IL-6 generation in mouse cementoblasts.
环加氧酶 2 依赖性前列腺素 E (2) 上调小鼠成牙骨质细胞中白细胞介素 (IL) -lalpha 诱导的 IL-6 生成。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Noguchi K;Miyauchi M;Oka H.;et. al.
- 通讯作者:et. al.
Isolation of multipotent stem cells from adult rat periodontal ligament by neurosphere-forming culture system.
- DOI:10.1016/j.bbrc.2007.04.031
- 发表时间:2007-06
- 期刊:
- 影响因子:3.1
- 作者:Wanida Techawattanawisal;K. Nakahama;M. Komaki;M. Abe;Y. Takagi;I. Morita
- 通讯作者:Wanida Techawattanawisal;K. Nakahama;M. Komaki;M. Abe;Y. Takagi;I. Morita
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KOMAKI Motohiro其他文献
KOMAKI Motohiro的其他文献
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{{ truncateString('KOMAKI Motohiro', 18)}}的其他基金
Elucidation of tissue regenerative mechanism caused by MSC derived-exosomes
阐明MSC衍生的外泌体引起的组织再生机制
- 批准号:
24659869 - 财政年份:2012
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Cell-patterning technology for epithelial rest of Malassez network and its application for periodontal regeneration
马拉塞斯网络上皮残留细胞图案化技术及其在牙周再生中的应用
- 批准号:
20592423 - 财政年份:2008
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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- 批准号:
19K19013 - 财政年份:2019
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