A genomic approach to the mechanism of meiotic recombination in Neurospora

在高等生物的生命周期中，在还原分裂过程中，重组使同源染色体之间的DNA序列发生改变。与突变一起，它是进化的关键过程。对重组分子过程的理解主要基于酵母，酵母不能容忍显著水平的序列错配，限制了正常重组事件分析的分辨率。我们已经证明，神经孢子虫，像其他不太容易处理的多细胞真核生物一样，能够容忍序列错配，允许对单个重组事件进行高分辨率分析。这个项目将建立在我们已经在理解重组如何发生方面取得的基本进展的基础上。

Recombination shuffles DNA sequences between homologous chromosomes during the reduction division in the life cycle of higher organisms. Along with mutation, it is a key process in evolution. Understanding of the molecular processes involved in recombination is largely based on yeast, which is intolerant of significant levels of sequence mismatch, limiting the resolution of analyses of normal recombination events. We have shown that Neurospora, like other less tractable multicellular eukaryotes, is tolerant of sequence mismatch, allowing high resolution analysis of individual recombination events. This project will build on fundamental advances we have already made in understanding how recombination occurs.