Plastic changes associated with spontaneous motor activity at neuromuscular junction.

与神经肌肉接头自发运动活动相关的塑性变化。

• 批准号: 19300140
• 负责人: KUROMI Hiroshi
• 金额: $ 11.9万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300140/
• 关键词: ニューロン・シナプス機能; ショウジョウバエ; 運動神経一筋接合部; シナプス伝達; シナプス小胞; シナプス可塑性; 小胞エンドサイトシス; 小胞リサイクル; リザーブプール; Ca2+チャネル; 神経筋シナプス; Ca^<2+>イメージング; Ca^<2+>チャネル

The present study revealed that (1) there were multiple Ca^<2+> channels at motor nerve terminals of Drosophila larvae. (2) After exocytosis of synaptic vesicles at nerve terminals, endocytosis, the first step of vesicle recycling, occurred at active zone and non-active zone linked to each specific CaCa^<2+> channels. (3) Both endocytosis contribute coordinately to maintain synaptic transmission, depending on degree of motor nerve activity. (4) In addition, changes in tubulin states, monomer or dimmer and their distribution, were associated with dynamics of synaptic vesicles. These results suggest that changes in synaptic vesicle traffic underlines plastic changes in synaptic transmission after strong activity of motor nerve.

结果表明：（1）果蝇幼虫运动神经末梢存在多个Ca^<2+>通道; (2)突触囊泡在神经末梢被胞吐后，在与各特异性CaCa^2+通道相连的活性区和非活性区发生胞吞作用，这是囊泡再循环的第一步。(3)这两种内吞作用协调地维持突触传递，这取决于运动神经活动的程度。(4)此外，微管蛋白状态的变化，单体或二聚体及其分布，与突触囊泡的动力学。这些结果表明，突触囊泡交通的变化强调了运动神经强烈活动后突触传递的可塑性变化。

项目成果

Nerve-evoked synchronous release and high K^+-induced quantal events are regulated separately by synaptotagmin I at Drosophila neuromuscular junction.

神经诱发的同步释放和高 K 诱导的量子事件由果蝇神经肌肉接头处的突触结合蛋白 I 分别调节。

• 发表时间: 2007
• 期刊: Journal of Neurophysiology 97
• 作者: Tamura;T.;Hou;J.;Reist;NE;Kidokoro.Y.
• 通讯作者: Kidokoro.Y.

Two types of Ca2+channel linked to two endocytic pathways coordinately maintain synaptic transmission at the Drosophila synapse

• DOI: 10.1111/j.1460-9568.2010.07300.x
• 发表时间: 2010-08-01
• 期刊: EUROPEAN JOURNAL OF NEUROSCIENCE
• 影响因子: 3.4
• 作者: Kuromi, H.;Ueno, K.;Kidokoro, Y.
• 通讯作者: Kidokoro, Y.

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Delayed Synaptic Transmission in Drosophila cacophonynull Embryos

• DOI: 10.1152/jn.90342.2008
• 发表时间: 2008-11-01
• 期刊: JOURNAL OF NEUROPHYSIOLOGY
• 影响因子: 2.5
• 作者: Hou, Jiamei;Tamura, Takuya;Kidokoro, Yoshiaki
• 通讯作者: Kidokoro, Yoshiaki