Plastic changes associated with spontaneous motor activity at neuromuscular junction.

结果表明：(1)果蝇幼虫运动神经末梢存在多种钙通道。(2)突触小泡在神经末梢排出后，囊泡循环的第一步--内吞作用发生在与每个特定的钙通道相连的活动区和非活动区。(3)两种内吞作用对维持突触传递有协同作用，这取决于运动神经活动的程度。(4)此外，微管蛋白状态、单体或二聚体及其分布的变化与突触小泡的动态变化有关。这些结果表明，突触小泡流量的变化突显了运动神经强烈活动后突触传递的可塑性变化。

The present study revealed that (1) there were multiple Ca^<2+> channels at motor nerve terminals of Drosophila larvae. (2) After exocytosis of synaptic vesicles at nerve terminals, endocytosis, the first step of vesicle recycling, occurred at active zone and non-active zone linked to each specific CaCa^<2+> channels. (3) Both endocytosis contribute coordinately to maintain synaptic transmission, depending on degree of motor nerve activity. (4) In addition, changes in tubulin states, monomer or dimmer and their distribution, were associated with dynamics of synaptic vesicles. These results suggest that changes in synaptic vesicle traffic underlines plastic changes in synaptic transmission after strong activity of motor nerve.