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Study of mechanism for synaptic vesicle recycling

突触小泡回收机制研究

基本信息

批准号：
18300131
负责人：
KUROMI Hiroshi
金额：
$ 10.12万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18300131/
关键词：
synaptic vesicles drosonhila endocytosis exocytosis recycle synaptic transmission active-zone non-active zone 神経終末 active-zone non-actve zone チュブリン synapse synaptic vesicles endocytosis La3+synaptic transmission

项目摘要

We have revealed four main findings on synaptic vesicle (SV) recycling in the period of April first, 2005 and March 31st, 2008. 1). There are two distinct pathways for endocytosis of SVs at the nerve terminals of Drosophila larvae and synaptic transmission at high frequency is maintained for a long time by two types of endocytosis which work coordinately. 2). Two types of endocytosis occurs at the active-zone and non-active zone at presynaptic nerve terminals. Two pathways are initiated by Ca2+ influx through two types of Cmaina2+ channels. Ca2+ channels which are encoded by straightjacket gene are identified as the channels which control active-zone endocytosis. 3). There has been no report to show the existence of tuburin at the nerve terminals. We produced transgenic flies which expressed GF-tubulin. GFP-tubulin changed their distribution in nerve terminals in response to nerve stimulation. Taxol, a tabilizer of tubulin, changed extents of endocytosis and traffic of SVs in nerve terminals. 4). After high activity of individual flie, synaptic transmission was enhanced by two steps. In the early phase, the quantum size increased and then the quantal contents increased.

在2005年4月1日至2008年3月31日期间，我们揭示了四个关于突触囊泡（SV）再循环的主要发现。1）。果蝇幼虫神经末梢的SV内吞有两种不同的途径，高频率的突触传递是通过两种类型的内吞协同工作而维持的。2）的情况。在突触前神经末梢的活动区和非活动区发生两种类型的内吞作用。Ca 2+通过两种类型的Cmaina 2+通道内流启动两条途径。由straightjacket基因编码的Ca 2+通道被认为是控制活性区内吞的通道。3）。还没有报道显示在神经末梢存在tuburin。我们产生了表达GF-微管蛋白的转基因果蝇。GFP-微管蛋白改变其在神经末梢中的分布以响应神经刺激。微管蛋白的稳定剂紫杉醇改变了神经末梢内吞和运输SV的程度。4）。在个体果蝇高度活动后，突触传递增强了两个步骤。在早期阶段，量子尺寸增加，然后量子含量增加。