Development of functional glyco-devices using fluorous interaction

利用氟相互作用开发功能性糖装置

• 批准号: 19300168
• 负责人: HATANAKA Kenichi
• 金额: $ 11.32万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300168/
• 关键词: 糖鎖; 糖鎖生産; 医用材料; 細胞; フルオラス; 糖鎖認識; 糖鎖プライマー; レクチン; ウイルス; インフルエンザ; GM3; 糖鎖の固定化; プライマー; グリコシル化; 臨界ミセル濃度; 表面張力; 細胞膜; フルオラスタグ

Glyco-compound having fluoroalkyl chain was synthesized and its carbohydrate chain was elongated using cells. The oligosaccharide having fluoroalkyl chain was immobilized on the fluoro-material and the immobilized material was tested for the absorption of influenza virus. It was found that the ethylene oxide oligomer was necessary as a spacer for recognition of influenza virus. It was confirmed that the immobilized oligosaccharide compound having oligo(ethylene oxide) spacer was specifically recognized by influenza virus.

合成了具有氟烷基链的糖化合物，并利用细胞延长了其碳水化合物链。将具有氟代烷基链的寡糖固定在氟材料上，并测试固定材料对流感病毒的吸收。发现环氧乙烷低聚物作为间隔物对于识别流感病毒是必需的。确认了具有寡聚(环氧乙烷)间隔基的固定化寡糖化合物被流感病毒特异性识别。

项目成果

酵素利用技術大系(細胞内糖鎖合成装置による糖脂質合成)

酶利用技术体系（利用细胞内糖链合成设备合成糖脂）

• 发表时间: 2010
• 作者: Q.D. Trinh;Y. Nanbu;T. Suzuki;S. Takahashi;M. Takeda;M. Kobayashi;畑中研一(分担執筆)
• 通讯作者: 畑中研一(分担執筆)

Lyso-GM3, its dimer, and multimer: their synthesis, and their effect on epidermal growth factor-induced receptor tyrosine kinase

• DOI: 10.1007/s10719-007-9051-2
• 发表时间: 2007-07
• 期刊: Glycoconjugate Journal
• 影响因子: 3
• 作者: Yoshimi Murozuka;Naoko Watanabe;K. Hatanaka;S. Hakomori

Purification of Gangliosides by Liquid-Liquid Partition Chromatography

液-液分配色谱法纯化神经节苷脂

• 发表时间: 2008
• 期刊: J.Lipid Res. Vol.49
• 作者: T.Kato;M.C.Kasuya;K.Hatanaka
• 通讯作者: K.Hatanaka

Cellular Uptake of Fluoroalkyl Glycoside and Saccharide-Chain Elongation

氟烷基糖苷的细胞摄取和糖链伸长

• 发表时间: 2008
• 作者: Keisuke Edashige;Delgado M. Valdez Jr.;Hiroshi Honda;Yu Nishikado;Bo Jin;Magosaburo Kasai;S.Nakano
• 通讯作者: S.Nakano

Incorporation of Fluorous Glycoside in Cell and Oligosaccharide Production

细胞和寡糖生产中掺入氟糖苷

• 发表时间: 2009
• 作者: 熊坂増高;武田元博;大内憲明;小林正樹;K.Hatanaka
• 通讯作者: K.Hatanaka